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Literature summary for 2.7.7.12 extracted from
- Galactose-1-phosphate uridylyltransferase: detection, isolation, and characterization of the uridylyl enzyme (1982), Methods Enzymol., 87, 20-36.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-galactose 1-phosphate | product/substrate inhibition | Escherichia coli |  |
| alpha-D-galactose 1-phosphate | - |
Homo sapiens | |
| alpha-D-glucose 1-phosphate | product/substrate inhibition | Escherichia coli | |
| alpha-D-glucose 1-phosphate | - |
Homo sapiens | |
| UDP-galactose | product/substrate inhibition | Escherichia coli | |
| UDP-glucose | product/substrate inhibition | Escherichia coli | |
| UDP-glucose | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| additional information | - |
additional information | kinetics | Homo sapiens | |
| 0.12 | - |
UDP-galactose | - |
Escherichia coli | |
| 0.14 | 0.16 | alpha-D-glucose 1-phosphate | - |
Escherichia coli | |
| 0.2 | 0.26 | UDP-glucose | pH 8.7, 25°C | Escherichia coli | |
| 0.2 | 0.26 | UDP-glucose | pH 8.7, 25°C | Saccharomyces cerevisiae | |
| 0.3 | 0.5 | alpha-D-galactose 1-phosphate | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride | Escherichia coli |
| 80000 | - |
low speed sedimentation equilibrium | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | Escherichia coli | 2nd step of Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | Homo sapiens | 2nd step of Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | Saccharomyces cerevisiae | 2nd step of Leloir pathway | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | ping pong bi bi kinetic model | Escherichia coli | |
| UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose | double-displacement mechanism | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | - |
Saccharomyces cerevisiae | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | 2nd step of Leloir pathway | Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | 2nd step of Leloir pathway | Homo sapiens | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
| UDP-glucose + alpha-D-galactose 1-phosphate | 2nd step of Leloir pathway | Saccharomyces cerevisiae | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Homo sapiens |
| dimer | - |
Saccharomyces cerevisiae |
| dimer | 2 * 41000, urea/mercaptoethanol treated and S-carboxymethylated enzyme, sedimentation equilibrium centrifugation in 5 M guanidine hydrochloride | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDPGlc:Gal-1-P uridylyltransferase | - |
Escherichia coli |
| UDPGlc:Gal-1-P uridylyltransferase | - |
Homo sapiens |
| UDPGlc:Gal-1-P uridylyltransferase | - |
Saccharomyces cerevisiae |
| UDPglucose-hexose-1-phosphate uridylyltransferase | - |
Escherichia coli |
| UDPglucose-hexose-1-phosphate uridylyltransferase | - |
Homo sapiens |
| UDPglucose-hexose-1-phosphate uridylyltransferase | - |
Saccharomyces cerevisiae |
| uridylylgalactose-1-P uridylyltransferase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Escherichia coli |
| 27 | - |
assay at | Saccharomyces cerevisiae |
| 27 | - |
both reaction directions | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
| 8.5 | - |
both reaction directions | Escherichia coli |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | product/substrate inhibition pattern and kinetics | Escherichia coli |
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