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Literature summary for 2.7.7.13 extracted from

  • Wu, B.; Zhang, Y.; Zheng, R.; Guo, C.; Wang, P.G.
    Bifunctional phosphomannose isomerase/GDP-D-mannose pyrophosphorylase is the point of control for GDP-D-mannose biosynthesis in Helicobacter pylori (2002), FEBS Lett., 519, 87-92.
    View publication on PubMed
Search for more literature for 2.7.7.13

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 alpha-D-mannose 1-phosphate pH 8.0, 37°C, cosubstrate GTP Helicobacter pylori
0.1
-
 GDPmannose pH 8.0, 37°C, cosubstrate diphosphate Helicobacter pylori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Helicobacter pylori
Mn2+ 30% of activity with Mg2+ Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Helicobacter pylori
55303
-
 x * 55303, deduced from nucleotide sequence Helicobacter pylori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate Helicobacter pylori enzyme regulates GDP-D-mannose synthesis through feedback inhibition GDPmannose + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, Ni-nitrilotriacetic acid column, Superdex 200 Helicobacter pylori

Reaction

Reaction Comment Organism Reaction ID
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose bifunctional enzyme with both phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activity Helicobacter pylori
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + alpha-D-mannose 1-phosphate
-
 Helicobacter pylori GDPmannose + diphosphate
-
 r
GTP + alpha-D-mannose 1-phosphate enzyme regulates GDP-D-mannose synthesis through feedback inhibition Helicobacter pylori GDPmannose + diphosphate
-
 r
GTP + alpha-D-mannose 1-phosphate
-
 Helicobacter pylori diphosphate + GDP-mannose
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Helicobacter pylori
? x * 55303, deduced from nucleotide sequence Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10.3
-
 alpha-D-mannose 1-phosphate pH 8.0, 37°C, cosubstrate GTP Helicobacter pylori
22.1
-
 GDPmannose pH 8.0, 37°C, cosubstrate diphosphate Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 GDP-mannose synthesis, forward reaction is very sensitive to pH fluctuation Helicobacter pylori
7 9 GTP synthesis Helicobacter pylori