Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | - |
Nitrosomonas europaea | |
phosphoenolpyruvate | - |
Nitrosomonas europaea | |
pyruvate | - |
Nitrosomonas europaea |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nitrosomonas europaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Nitrosomonas europaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, presence of Mg2+ | Nitrosomonas europaea | |
0.13 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, presence of Co2+ | Nitrosomonas europaea | |
0.13 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, presence of Mn2+ | Nitrosomonas europaea | |
0.72 | - |
alpha-D-glucose 1-phosphate | pH 8.0, 37°C, presence of Cd2+ | Nitrosomonas europaea |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | S0.5 value 0.74 mM in direction of synthesis of ADP-glucose | Nitrosomonas europaea | |
Co2+ | S0.5 value 0.46 mM in direction of synthesis of ADP-glucose | Nitrosomonas europaea | |
Mg2+ | S0.5 value above 40 mM in direction of synthesis of ADP-glucose | Nitrosomonas europaea | |
Mn2+ | S0.5 value 1.37 mM in direction of synthesis of ADP-glucose | Nitrosomonas europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
4 * 49200, calculated, 4 * 49000, SDS-PAGE | Nitrosomonas europaea |
49200 | - |
4 * 49200, calculated, 4 * 49000, SDS-PAGE | Nitrosomonas europaea |
200000 | - |
gel filtration | Nitrosomonas europaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrosomonas europaea | Q82T88 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Nitrosomonas europaea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
pH 8.0, 37°C | Nitrosomonas europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + alpha-D-glucose 1-phosphate | the catalytic efficiency for ATP is not dependent upon the divalent metal ion | Nitrosomonas europaea | diphosphate + ADP-glucose | - |
r | |
ATP + alpha-D-mannose 1-phosphate | the catalytic efficiency with glucose 1-phosphate is at least one order of magnitude higher than that with mannose 1-phosphate | Nitrosomonas europaea | diphosphate + ADP-mannose | - |
r | |
additional information | enzyme is able to accept UTP, CTP, dTTP and GTP with catalytic efficiencies below 1% of that with ATP | Nitrosomonas europaea | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 49200, calculated, 4 * 49000, SDS-PAGE | Nitrosomonas europaea |
Synonyms | Comment | Organism |
---|---|---|
GlgC | - |
Nitrosomonas europaea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | fully active between 0°C and 50°C for 15 min | Nitrosomonas europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Nitrosomonas europaea |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.8 | 9.1 | stable for 30 min at 37°C | Nitrosomonas europaea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Nitrosomonas europaea | calculated | - |
5.8 |