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Literature summary for 2.7.7.31 extracted from

  • Kaneda, T.; Kuroda, S.; Koiwai, O.; Yoshida, S.
    Purification of terminal deoxynucleotidyl transferase from pig thymus: identification of 42,000 and 57,000 dalton species (1981), J. Biochem., 90, 1421-1427.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP
-
Sus scrofa
N-ethylmaleimide
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
oligo(dA)12-18 37°C, pH 8.3 Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
1 * 42000, low-MW form, SDS-PAGE Sus scrofa
45000
-
2 MW forms: 45000 and 57000, gel filtration Sus scrofa
57000
-
2 MW forms: 45000 and 57000, gel filtration Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 MW forms: 45000 and 57000 Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn primer required Sus scrofa diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn reaction is not template directed Sus scrofa diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn deoxynucleoside triphosphate preference: dGTP, dCTP, dTTP, dATP (42000 MW enzyme) Sus scrofa diphosphate + DNAn+1
-
?
dGTP + oligo(dA)12-18
-
Sus scrofa ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 57000, high-MW form, SDS-PAGE Sus scrofa
monomer 1 * 42000, low-MW form, SDS-PAGE Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa