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Literature summary for 2.7.7.35 extracted from
- Phosphorolysis of adenosine diphosphoribose (1966), Arch. Biochem. Biophys., 113, 236-244.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-ribose | competitive to phosphate | Euglena gracilis |  |
| Ag2+ | - |
Euglena gracilis | |
| D-ribose 5-phosphate | competitive to phosphate with ADP, noncompetitive to phosphate with ADP-ribose | Euglena gracilis | |
| deoxyribose 5-phosphate | ADP/phosphate-exchange | Euglena gracilis | |
| additional information | no inhibition by CN-, 1,10-phenanthroline, 2,2'-dipyridyl, ribose 1-phosphate, ribose 1,5-diphosphate, glucose 1-phosphate, glucose 6-phosphate; no inhibition by NADP+, EDTA, ribose | Euglena gracilis | |
| PCMB | ADP/phosphate-exchange, reversible by cysteine | Euglena gracilis | |
| SeO42- | - |
Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Euglena gracilis | |
| 0.04 | - |
ADP-ribose | pH 7.8, 30°C | Euglena gracilis | |
| 0.4 | - |
phosphate | pH 7.8, 30°C | Euglena gracilis | |
| 0.6 | - |
ADP | pH 7.8, 30°C | Euglena gracilis | |
| 0.6 | - |
ADP | ADP/phosphate exchange | Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Euglena gracilis | 5829 | - |
| cytosol | - |
Ochromonas danica | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no metal ion requirement | Euglena gracilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + ADP-ribose | Euglena gracilis | - |
ADP + D-ribose 5-phosphate | - |
ir | |
| phosphate + ADP-ribose | Ochromonas danica | - |
ADP + D-ribose 5-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
green alga, strain Z | - |
| no activity in Chlorella sp. | - |
- |
- |
| no activity in Gloeocapsa sp. | - |
- |
- |
| no activity in Pisum sativum | - |
- |
- |
| no activity in Spinacia oleracea | - |
- |
- |
| Ochromonas danica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Euglena gracilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ADP + D-ribose 5-phosphate = phosphate + ADP-D-ribose | ping-pong reaction mechanism | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Ochromonas danica | - |
| cell culture | heterotrophically or autotrophically grown cells | Euglena gracilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
low activity | Ochromonas danica |
| 1.3 | - |
partially purified enzyme | Euglena gracilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, fairly stable in the presence of EDTA | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose | Euglena gracilis | ? | - |
? | |
| additional information | no activity with crude polyadenylic acid, RNA from Euglena, and NAD(H), NADP(H), coenzyme A, FAD | Euglena gracilis | ? | - |
? | |
| phosphate + ADP | IDP can replace ADP with 16% efficiency | Euglena gracilis | ADP + phosphate | - |
r | |
| phosphate + ADP | IDP can replace ADP with 16% efficiency | Ochromonas danica | ADP + phosphate | - |
r | |
| phosphate + ADP-ribose | - |
Euglena gracilis | ADP + D-ribose 5-phosphate | - |
ir | |
| phosphate + ADP-ribose | - |
Ochromonas danica | ADP + D-ribose 5-phosphate | - |
? | |
| phosphate + ADP-ribose | - |
Ochromonas danica | ADP + D-ribose 5-phosphate | - |
ir | |
| phosphate + ADP-ribose | highly specific | Euglena gracilis | ADP + D-ribose 5-phosphate | - |
ir | |
| phosphate + deamino-NAD+ | inosine diphosphoribose, not adenosine triphosphoribose | Euglena gracilis | ? | - |
? | |
| phosphate + deamino-NAD+ | inosine diphosphoribose, not adenosine triphosphoribose | Ochromonas danica | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 7.8 | ADP/phosphate-exchange | Euglena gracilis |
| 8 | - |
ADP-ribose phosphorolysis | Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8.1 | ADP/phosphate-exchange, about half-maximal activity at pH 6.4 and pH 8.1 | Euglena gracilis |
| 6.8 | 9 | ADP-ribose phosphorolysis, about half-maximal activity at pH 6.8 and about 90% of maximal activity at pH 9.0 | Euglena gracilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
D-ribose 5-phosphate | pH 7.8, 30°C | Euglena gracilis | |
| 0.11 | - |
D-ribose 5-phosphate | ADP/phosphate-exchange reaction | Euglena gracilis | |
| 4 | - |
D-ribose 5-phosphate | phosphorolysis, pH 7.8, 30°C | Euglena gracilis | |
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Release 2023.1 (January 2023)
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