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Literature summary for 2.7.7.4 extracted from
- ATP sulfurylase from the hyperthermophilic chemolithotroph Aquifex aeolicus (2002), Arch. Biochem. Biophys., 406, 275-288.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL-21 | Aquifex aeolicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ClO3- | - |
Aquifex aeolicus |  |
| ClO4- | competitive with sulfate and adenylyl sulfate | Aquifex aeolicus | |
| FSO3- | - |
Aquifex aeolicus | |
| NO3- | - |
Aquifex aeolicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0051 | - |
adenosine 5'-phosphosulfate | pH 8.0, 37°C | Aquifex aeolicus | |
| 0.057 | - |
diphosphate | pH 8.0, 30°C | Aquifex aeolicus | |
| 0.15 | - |
MoO42- | pH 8.0, 30°C | Aquifex aeolicus | |
| 0.23 | - |
MgATP2- | pH 8.0, 30°C, reaction with MoO42- | Aquifex aeolicus | |
| 1.1 | - |
MgATP2- | pH 8.0, 30°C, reaction with SO42- | Aquifex aeolicus | |
| 3.3 | - |
SO42- | pH 8.0, 30°C | Aquifex aeolicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62800 | - |
4 * 62800, calculation from amino acid sequence | Aquifex aeolicus |
| 260000 | - |
gel filtration | Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Aquifex aeolicus | the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O67174 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MoO42- | - |
Aquifex aeolicus | AMP + adenylylmolybdate | - |
? | |
| ATP + sulfate | - |
Aquifex aeolicus | diphosphate + adenylylsulfate | - |
r | |
| additional information | the enzyme may also function to produce 3'-phosphoadenosine 5'-phosphosulfate for sulfate ester formation or sulfate assimilation | Aquifex aeolicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62800, calculation from amino acid sequence | Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
half-life: more than 1 h | Aquifex aeolicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Aquifex aeolicus |
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