Literature summary for 2.7.7.4 extracted from

Gavel, O.Y.; Bursakov, S.A.; Calvete, J.J.; George, G.N.; Moura, J.J.G.; Moura, I.
ATP Sulfurylases from sulfate-reducing bacteria of the genus Desulfovibrio. A novel metalloprotein containing cobalt and zZinc (1998), Biochemistry, 37, 16225-16232.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cobalt	metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms	Desulfovibrio desulfuricans
Cobalt	metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms	Megalodesulfovibrio gigas
Cobalt	contains 1.53 mol of zinc per mol of protein	Megalodesulfovibrio gigas
Cobalt	contains 1.89 mol of zinc per mol of protein	Desulfovibrio desulfuricans
Zinc	contains 0.72 mol of zinc per mol of protein	Desulfovibrio desulfuricans
Zinc	contains 1.38 mol of zinc per mol of protein	Megalodesulfovibrio gigas
Zinc	metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms	Desulfovibrio desulfuricans
Zinc	metalloprotein. Either cobalt or zinc binds endogenously at presumably equivalent metal binding sites and is tetrahedrally coordinated to one nitrogen and three sulfur atoms	Megalodesulfovibrio gigas

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
47100	-	3 * 47100, electrospray mass spectrometry	Desulfovibrio desulfuricans
138000	-	gel filtration	Desulfovibrio desulfuricans
151000	-	gel filtration	Megalodesulfovibrio gigas

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + sulfate	Desulfovibrio desulfuricans	-	diphosphate + adenylylsulfate	-	r
ATP + sulfate	Megalodesulfovibrio gigas	enzyme plays a crucial role in sulfate activation	diphosphate + adenylylsulfate	-	r

Organism

Organism	UniProt	Comment	Textmining
Desulfovibrio desulfuricans	-	ATCC 27774	-
Megalodesulfovibrio gigas	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Desulfovibrio desulfuricans
-	Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
22.5	-	-	Desulfovibrio desulfuricans
28.9	-	-	Megalodesulfovibrio gigas

Storage Stability

Storage Stability	Organism
4°C, 50 mM MOPS buffer, pH 7.4, 0.2 mM CoCl2, 0.2 mM ZnCl2, stable for 48 h	Desulfovibrio desulfuricans
4°C, 50 mM MOPS buffer, pH 7.4, 0.2 mM CoCl2, 0.2 mM ZnCl2, stable for 48 h	Megalodesulfovibrio gigas

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + sulfate	-	Desulfovibrio desulfuricans	diphosphate + adenylylsulfate	-	r
ATP + sulfate	-	Megalodesulfovibrio gigas	diphosphate + adenylylsulfate	-	r
ATP + sulfate	enzyme plays a crucial role in sulfate activation	Megalodesulfovibrio gigas	diphosphate + adenylylsulfate	-	r

Subunits

Subunits	Comment	Organism
trimer	3 * 47100, electrospray mass spectrometry	Desulfovibrio desulfuricans
trimer	3 * 48000-50000, electrospray mass spectrometry	Megalodesulfovibrio gigas

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Release 2023.1 (January 2023)