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Literature summary for 2.7.7.4 extracted from

  • Hanna, E.; Ng, K.F.; MacRae, I.J.; Bley, C.J.; Fisher, A.J.; Segel, I.H.
    Kinetic and stability properties of Penicillium chrysogenum ATP sulfurylase missing the C-terminal regulatory domain (2004), J. Biol. Chem., 279, 4415-4424.
    View publication on PubMed
Search for more literature for 2.7.7.4

Cloned(Commentary)

Cloned (Comment) Organism
truncated enzyme form Penicillium chrysogenum

Protein Variants

Protein Variants Comment Organism
del396-573 recombinant ATP sulfurylase lacking the C-terminal allosteric domain is monomeric and noncooperative. Mutant enzyme is less heat stable than wild-type enzyme. Wild-type enzyme behaves cooperative at pH 6.5, truncated enzyme form displays normal hyperbolic behavior Penicillium chrysogenum

Inhibitors

Inhibitors Comment Organism Structure
3'-phosphoadenosine 5'-phosphosulfate allosteric, binding of the inhibitor to the catalytic site as well as to the allosteric site of the wild type enzyme acts to decrease the degree of cooperativity Penicillium chrysogenum
5,5'-dithiobis(2-nitrobenzoic acid) 0.05 mM, rapid decrease in activity of wild-type enzyme (t1/2: 20 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min Penicillium chrysogenum
adenosine 5'-phosphosulfate competitive with both substrates Penicillium chrysogenum
diphosphate noncompetitive with respect to MgATP and sulfate Penicillium chrysogenum
FSO3- 0.03 mM, 50% inhibition Penicillium chrysogenum
NEM 0.15 mM, rapid decrease in activity of wild-type enzyme (t1/2: 45 s), truncated enzyme del396-573 retains more than 97% of its activity after 30 min Penicillium chrysogenum
Phenylglyoxal 3 mM, irreversible inactivation of wild-type enzyme and mutant enzyme del396-573, t1/2: 5 min for both forms Penicillium chrysogenum
thiosulfate competitive with molybdate and noncompetitive with MgATP Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 adenylylsulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
0.0005
-
 adenylylsulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
0.0092
-
 diphosphate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
0.025
-
 diphosphate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
0.027
-
 ATP pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 Penicillium chrysogenum
0.027
-
 ATP pH 8.0, 30°C, molybdolysis, wild-type enzyme Penicillium chrysogenum
0.076
-
 MoO42- pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
0.21
-
 ATP pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme Penicillium chrysogenum
0.29
-
 sulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
0.53
-
 MoO42- pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
2.6
-
 ATP pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 Penicillium chrysogenum
3.6
-
 sulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum Q12650
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + MoO42-
-
 Penicillium chrysogenum AMP + adenylylmolybdate
-
 r
ATP + sulfate
-
 Penicillium chrysogenum diphosphate + adenylylsulfate
-
 ?

Subunits

Subunits Comment Organism
hexamer wild-type enzyme Penicillium chrysogenum
monomer 1 * 46000,truncated mutant enzyme del396-573, SDS-PAGE Penicillium chrysogenum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 t1/2 for mutant enzyme del396-573: 1.5 min Penicillium chrysogenum
50
-
 t1/2 for mutant enzyme del396-573: 0.3 min, wild-type enzyme is stable for more than 2 h Penicillium chrysogenum
62
-
 t1/2 for wild-type enzyme: 1.5 min Penicillium chrysogenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 ATP pH 8.0, 30°C, synthesis of adenylylsulfate, truncated mutant enzyme del396-573 Penicillium chrysogenum
1.8
-
 sulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
10.8
-
 ATP pH 8.0, 30°C, synthesis of adenylylsulfate, wild-type enzyme Penicillium chrysogenum
10.8
-
 sulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
13.7
-
 ATP pH 8.0, 30°C, molybdolysis, truncated mutant enzyme del396-573 Penicillium chrysogenum
13.7
-
 MoO42- pH 8.0, 30°C,truncated mutant enzyme del396-573 Penicillium chrysogenum
24.4
-
 ATP pH 8.0, 30°C, molybdolysis, wild-type enzyme Penicillium chrysogenum
24.4
-
 MoO42- pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
46.6
-
 adenylylsulfate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
46.6
-
 adenylylsulfate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum
73.3
-
 diphosphate pH 8.0, 30°C, truncated mutant enzyme del396-573 Penicillium chrysogenum
73.3
-
 diphosphate pH 8.0, 30°C, wild-type enzyme Penicillium chrysogenum