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Literature summary for 2.7.7.4 extracted from

  • Phartiyal, P.; Kim, W.S.; Cahoon, R.E.; Jez, J.M.; Krishnan, H.B.
    Soybean ATP sulfurylase, a homodimeric enzyme involved in sulfur assimilation, is abundantly expressed in roots and induced by cold treatment (2006), Arch. Biochem. Biophys., 450, 20-29.
    View publication on PubMed

Application

Application Comment Organism
agriculture increasing the expression level of this key enzyme during soybean seed development could lead to an increase in the availability of sulfur amino acids, thereby enhancing the nutritional value of the crop Glycine max

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli as a truncated version of soybean ATPS lacking the first 48 amino acids, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli Glycine max
the protein is insoluble when the full-length cDNA of soybean ATPS, including the chloroplast/plastid localisation sequence, is used for expression in Escherichia coli Glycine max

Protein Variants

Protein Variants Comment Organism
DELTA48 a truncated version of soybean ATPS lacking the first 48 amino acids is generated for protein expression and purification, removal of the putative localisation sequence improves the yield of N-terminally His-tagged protein in Escherichia coli Glycine max
additional information the plant enzymes only contain ATP sulfurylase domain, unlike the human and yeast enzymes which include an APS kinase domain, located at the N- or C-terminal regions, respectively Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00211
-
sulfate forward reaction, APS synthesis Glycine max
0.0191
-
diphosphate reverse reaction, ATP synthesis Glycine max
0.0191
-
diphosphate diphosphate in form of magnesium diphosphate Glycine max
0.0245
-
adenylyl sulfate reverse reaction, ATP synthesis Glycine max
0.195
-
ATP forward reaction, APS synthesis Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ for APS synthesis assay contains 15 mM MgCl2, for ATP synthesis assay contains 5 mM MgCl2 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 Glycine max
50000
-
enzyme functions as a 2 * 50000 Da homodimer Glycine max
52000
-
predicted from cDNA sequence, enzyme functions as a 100 kDa homodimer Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max Q8SAG1
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26 Glycine max

Purification (Commentary)

Purification (Comment) Organism
N-terminal His-tagged protein is passed over a Ni2+-NTA column Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-
additional information by northern blot analysis a decline in the ATPS transcript level during seed development can be found Glycine max
-
root ATPS mRNA is most abundant in root tissue, cold treatment induces RNA accumulation and enhances the specific activity of ATPS in root tissue Glycine max
-
seed
-
Glycine max
-
stem
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
ATPS activity gradually increases during cold treatment, after 72h at 10°C ATPS activity is 2.2fold higher Glycine max
additional information
-
ATPS activity in stem and leaf tissue are comparable but lower than in seeds and roots Glycine max
additional information
-
ATPS activity in young seeds is several-folds higher than in any other tissue Glycine max
2.9
-
specific activity for APS synthesis Glycine max
48.7
-
specific activity for ATP synthesis Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfate
-
Glycine max diphosphate + adenylyl sulfate
-
r
diphosphate + adenylyl sulfate
-
Glycine max ATP + sulfate
-
r
diphosphate + adenylyl sulfate diphosphate in form of magnesium diphosphate Glycine max ATP + sulfate
-
r

Subunits

Subunits Comment Organism
homodimer enzyme functions as a 2 * 50000 Da homodimer Glycine max

Synonyms

Synonyms Comment Organism
ATP sulfurylase
-
Glycine max
ATPS
-
Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.49
-
ATP forward reaction, APS synthesis Glycine max
2.11
-
sulfate forward reaction, APS synthesis Glycine max
19.1
-
diphosphate reverse reaction, ATP synthesis Glycine max
24.5
-
adenylyl sulfate reverse reaction, ATP synthesis Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Glycine max

pI Value

Organism Comment pI Value Maximum pI Value
Glycine max ATPS contains a chloroplast/plastid transit peptide sequence in its N-terminal region, processing of the transit peptide releases a mature protein of 46.4 kDa an a pI of 6.26
-
additional information
Glycine max predicted from cDNA sequence
-
7.3