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Literature summary for 2.7.7.4 extracted from

  • Ravilious, G.; Herrmann, J.; Lee, S.; Westfall, C.; Jez, J.
    Kinetic mechanism of the dimeric ATP sulfurylase from plants (2013), Biosci. Rep., 33, 585-591.
No PubMed abstract available
Search for more literature for 2.7.7.4

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli BL21(DE3) Glycine max

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + sulfate Glycine max
-
 diphosphate + adenylyl sulfate
-
 r

Organism

Organism UniProt Comment Textmining
Glycine max Q8SAG1 ATP sulfurylase isoform 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration Glycine max

Reaction

Reaction Comment Organism Reaction ID
ATP + sulfate = diphosphate + adenylyl sulfate analysis of the bi bi substrate kinetic mechanism of GmATPS1. The enzyme shows a kinetic mechanism in which ATP and APS are the first substrates bound in the forward and reverse reactions, respectively Glycine max
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + sulfate
-
 Glycine max diphosphate + adenylyl sulfate
-
 r
additional information no binding of diphosphate to enzyme GmATPS1 Glycine max ?
-
 ?

Synonyms

Synonyms Comment Organism
ATP sulfurylase
-
 Glycine max
ATP sulfurylase isoform 1
-
 Glycine max
ATP: sulfate adenylyl transferase
-
 Glycine max
ATPS1
-
 Glycine max

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Glycine max

General Information

General Information Comment Organism
evolution in plants, gene families encode multiple isoforms of ATP sulfurylase with varied expression patterns and organelle localization Glycine max
metabolism the first enzymatic reaction in the sulfur assimilation pathway of plants is the non-reductive adenylation of sulfate catalysed by ATP sulfurylase to yield adenylyl sulfate, APS, and diphosphate. The enzyme also catalyzes the next step, producing ADP and 3'-phosphoadenylyl sulfate from ATP and adenylyl sulfate, cf. EC 2.7.1.25 Glycine max
physiological function the sulfur assimilation pathway provides sulfide for a range of biosynthetic pathways that supply methionine, glutathione, iron–sulfur clusters, vitamin cofactors such as biotin and thiamin, and multiple specialized metabolites such as glucosinolates Glycine max