General Stability | Organism |
---|---|
stable to at least 2 cycles of freezing and thawing | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dCTP | competitive with respect to CTP | Saccharomyces cerevisiae | |
Thiophosphatidate | competitive | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
phosphatidate | - |
Saccharomyces cerevisiae | |
1 | - |
CTP | pH 6.5, 30°C | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | about 20% of enzyme is associated with | Saccharomyces cerevisiae | - |
- |
mitochondrion | main site of localization | Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
114000 | - |
- |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mitochondrial enzyme | Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + phosphatidate = diphosphate + CDP-diacylglycerol | sequential bi-bi reaction: CDPdiacylglycerol synthase binds to CTP prior to phosphatidate and diphosphate is released prior to CDPdiacylglycerol in the reaction sequence | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-80°C, in presence of 1 mM CTP the purified enzyme is 90-100% stable for at least 3 months | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | - |
Saccharomyces cerevisiae | diphosphate + CDPdiacylglycerol | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
20 min, labile above | Saccharomyces cerevisiae |