Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulates only in presence of either lecithin or sphingomyelin | Sus scrofa | |
cationic detergent | the phosphatidate must be emulsified in a cationic detergent for optimal activity | Sus scrofa | |
GTP | stimulates only in presence of either lecithin or sphingomyelin | Sus scrofa | |
ITP | stimulates only in presence of either lecithin or sphingomyelin | Sus scrofa | |
lecithin | stimulates | Sus scrofa | |
sphingomyelin | stimulates | Sus scrofa | |
UTP | stimulates only in presence of either lecithin or sphingomyelin | Sus scrofa |
General Stability | Organism |
---|---|
-20°C, enzyme is very unstable when the disrupted lymphocyte homogenate or the isolated microsomes are stored, 75% loss of activity after 24 h. Stable for at least 1 month if stored in intact lymphocytes | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 45% inhibition, even in presence of optimal Mg2+ concentrations | Sus scrofa | |
cardiolipin | 2 mM, 27% inhibition | Sus scrofa | |
Co2+ | 2 mM, 58% inhibition, even in presence of optimal Mg2+ concentrations | Sus scrofa | |
F- | 20 mM NaF, 70% inhibition | Sus scrofa | |
FeSO4 | 2 mM, 97% inhibition, even in presence of optimal Mg2+ concentrations | Sus scrofa | |
lysolecithin | 1 mM, 89% inhibition | Sus scrofa | |
oleic acid | 2 mM, 84% inhibition | Sus scrofa | |
palmitic acid | 2 mM, 76% inhibition | Sus scrofa | |
palmitoyl-CoA | 0.5 mM, 70% inhibition | Sus scrofa | |
phosphatidylethanolamine | 2 mM, 16% inhibition | Sus scrofa | |
phosphatidylinositol | 2 mM, 23% inhibition | Sus scrofa | |
phosphatidylserine | 2 mM, 46% inhibition | Sus scrofa | |
SDS | - |
Sus scrofa | |
sodium deoxycholate | - |
Sus scrofa | |
Triton X-100 | - |
Sus scrofa | |
Tween 20 | - |
Sus scrofa | |
ZWT-12 | 2 mM, 86% inhibition | Sus scrofa | |
ZWT-14 | 2 mM, 92% inhibition | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
CTP | pH 7.0, 37°C | Sus scrofa | |
0.65 | - |
phosphatidate | pH 7.0, 37°C | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal stimulation at 20 mM | Sus scrofa | |
Mn2+ | up to 10 mM, never more than 15% as effective as Mg2+ | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
hog | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphocyte | - |
Sus scrofa | - |
mesenteric lymph node | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00011 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, 24 h, enzyme in isolated microsomes, 75% loss of activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + phosphatidate | activity with phosphatidate obtained from egg lecithin which has a variety of mixed fatty acid in ester linkage. The phosphatidate has two oleoyl fatty acids is only 20% as effective as the phospatidate prepared from egg lecithin | Sus scrofa | diphosphate + CDPdiacylglycerol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Tris-HCl buffer | Sus scrofa |
7.5 | - |
phosphate buffer | Sus scrofa |