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Literature summary for 2.7.7.45 extracted from
- GTP:GTP guanylyl transferase: trapping procedures for detecting and characterizing chemical nature of enzyme-nucleotide phosphoramidate reaction intermediate (2002), Methods Enzymol., 354, 251-260.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
x * 110000, SDS-PAGE | Artemia franciscana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + GTP | Artemia franciscana | - |
diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r |  |
| GTP + GTP | Daphnia magna | - |
diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Artemia franciscana | - |
- |
- |
| Daphnia magna | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Artemia franciscana |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 2 GTP = diphosphate + P1,P4-bis(5'-guanosyl) tetraphosphate | the alpha-phosphorus group of the GMP of the covalent reaction intermediate is linked via the Nepsilon2 ring nitrogen of a histidine residue of the enzyme | Artemia franciscana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cyst | - |
Artemia franciscana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + GTP | - |
Daphnia magna | P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate | i.e. GP4G | r | |
| GTP + GTP | the alpha-phosphorus group of the GMP of the covalent reaction intermediate is linked to the Nepsilon2 ring nitrogen of a histidine residue of the enzyme | Artemia franciscana | P1,P4-bis(5'-guanosyl)tetraphosphate + diphosphate | i.e. GP4G | r | |
| GTP + GTP | - |
Artemia franciscana | diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r | |
| GTP + GTP | - |
Daphnia magna | diphosphate + P1,P4-bis(5'-guanosyl)tetraphosphate | - |
r | |
| GTP + ITP | - |
Artemia franciscana | GppppI + diphosphate | - |
r | |
| GTP + XTP | - |
Artemia franciscana | GppppX + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 110000, SDS-PAGE | Artemia franciscana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Artemia franciscana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
20 min, 4 M sodium acetate, pH 7.5, 96% of maximal activity | Artemia franciscana |
| 37 | - |
20 min, 3.68 M hydroxylamine, pH 4.75, 0.7% of maximal activity | Artemia franciscana |
| 37 | - |
20 min, 0.2 M hydroxylamine, pH 7.5, 2.5% of maximal activity | Artemia franciscana |
| 85 | - |
5 min: stable at alkaline conditions of 0.15 M NaOH, 79.1% of maximal activity at pH 7.5, 1% of maximal activity at acidic conditions of 0.15 M HCl | Artemia franciscana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
stable at alkaline pH conditions at 85% for 5 min | Artemia franciscana |
| additional information | - |
unstable at acidic pH conditions | Artemia franciscana |
| additional information | - |
stability of the enzyme-guanylate reaction intermediate complex at different temperatures and pH values | Artemia franciscana |
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Release 2023.1 (January 2023)
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