Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | competitive with GTP | Qubevirus durum | |
ppGpp | competitive with GTP | Qubevirus durum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
GTP | 30°C | Qubevirus durum | |
0.02 | - |
GTP | 30°C, cross-linked enzyme | Qubevirus durum |
Organic Solvent | Comment | Organism |
---|---|---|
urea | denaturation | Qubevirus durum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Qubevirus durum | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
when the enzyme is denatured with 8 M urea, the rate of its renaturation following dilution in a high-salt buffer is inhibited by relatively low concentrations of GDP, ppGpp and GTP | Qubevirus durum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNAn | - |
Qubevirus durum | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
Qubevirus durum | diphosphate + RNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
60 min, 40-60% loss of poly(G) polymerase activity, in presence of 2 mM guanine nucleotides loss of activity is up to 91% of the original specific activity | Qubevirus durum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
GDP | 30°C | Qubevirus durum | |
0.25 | - |
ppGpp | 30°C | Qubevirus durum |