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Literature summary for 2.7.7.48 extracted from
- Isolation and characterization of an RNA-dependent RNA polymerase from Black beetle virus-infected Drosophila melanogaster cells (1981), J. Virol., 40, 379-386.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cl- | - |
black beetle virus |  |
| Mn2+ | above 2 mM | black beetle virus | |
| RNase | pancreatic RNAse and T1 RNAse | black beetle virus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
UTP | pH 8.2, 29°C | black beetle virus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | particulate material of cells of Drosophila melanogaster infected with Black beetle virus | black beetle virus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight stimulation by potassium acetate, optimal activity at 60 mM | black beetle virus | |
| Mg2+ | required, optimal activity with 10-20 mM magnesium acetate | black beetle virus | |
| Mn2+ | Mg2+ requirement can be partially replaced by Mn2+ | black beetle virus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| black beetle virus | - |
Drosophila melanogaster infected with | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
black beetle virus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | cells of Drosophila melanogaster infected with Black beetle virus | black beetle virus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00000016 | - |
- |
black beetle virus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for several months | black beetle virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | full-length negative strand BBV RNAs are synthesized | black beetle virus | diphosphate + RNAn+1 | - |
? | |
| UTP + RNAn | - |
black beetle virus | diphosphate + RNAn+1 | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 29 | - |
- |
black beetle virus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 18 | 37 | 18°C: about 50% of maximal activity, 37°C: about 50% of maximal activity | black beetle virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
- |
black beetle virus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 9 | pH 7.6: about 70% of maximal activity, pH 9.0: about 80% of maximal activity | black beetle virus |
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