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Literature summary for 2.7.7.48 extracted from

  • Gohara, D.W.; Crotty, S.; Arnold, J.J.; Yoder, J.D.; Andino, R.; Cameron, C.E.
    Poliovirus RNA-dependent RNA polymerase (3Dpol): structural, biochemical, and biological analysis of conserved structural motifs A and B (2000), J. Biol. Chem., 275, 25523-25532.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D238A poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.24% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 1.9% of the activity of the wild-type enzyme Enterovirus C
D238E poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.04% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.74% of the activity of the wild-type enzyme Enterovirus C
D238F poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 4.4% of the activity of the wild-type enzyme. No AMP incorporation activity in presence of Mn2+ or Mg2+ Enterovirus C
D238N poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 45% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 0.05% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.797% of the activity of the wild-type enzyme Enterovirus C
D238V poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 11% of the activity of the wild-type enzyme. No AMP incorporation activity in presence of Mn2+ or Mg2+ Enterovirus C
N297A poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 89% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 11.2% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 4.5% of the activity of the wild-type enzyme Enterovirus C
N297D poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is 89% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 38.2% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 21.2% of the activity of the wild-type enzyme Enterovirus C
N297Q poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is about 35% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 1.4% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 0.72% of the activity of the wild-type enzyme Enterovirus C
N297V poly(rU) polymerase activity determined by using dT15/rA30 in presence of Mn2+ is about 55% of the activity of the wild-type enzyme. AMP incorporation in presence of Mg2+, at 0.1 mM ATP, is 15.3% of the activity of the wild-type enzyme. AMP incorporation in presence of Mn2+, at 0.1 mM ATP, is 4.9% of the activity of the wild-type enzyme Enterovirus C

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Enterovirus C
Mn2+ stimulates to a higher extent than Mg2+, poly(rU) polymerase activity using dT15/rA30 Enterovirus C

Organism

Organism UniProt Comment Textmining
Enterovirus C
-
 expressed in Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
nucleoside triphosphate + RNAn
-
 Enterovirus C diphosphate + RNAn+1
-
 ?