Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pCG1 cells | Enterovirus C |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | 100 nM heparin rapidly inactivates 3Dpol by inhibiting reinitiation | Enterovirus C | |
NTP | 3Dpol is inhibited by NTP concentrations higher than 2 mM | Enterovirus C |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterovirus C | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose SPHP XK16/20 column chromatography, Q20 column chromatography, Q5 column chromatography, and UNO column chromatography | Enterovirus C |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Enterovirus C | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | RdRP incorporation of incorrect nucleosides is inefficient, making precise determination of kinetic parameters experimentally challenging. The fidelity for poliovirus polymerase 3Dpol ranges from 12000 to 1000000 for transition mutations and 32000 to 43000000 for transversion mutations | Enterovirus C | diphosphate + RNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3Dpol | - |
Enterovirus C |
polymerase 3Dpol | - |
Enterovirus C |
RDRP | - |
Enterovirus C |
RNA-dependent RNA polymerase | - |
Enterovirus C |