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Literature summary for 2.7.7.48 extracted from
- RNA polymerases (2008), Curr. Protoc. Mol. Biol., Chapter 3, Unit3.8.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | RdRp from the bacteriophage phi6 can be used to generate dsRNA for RNA interference, RNAi, applications, resulting in in vivo gene silencing. RNA molecules that are radioactively or fluorescently labeled using poly(A) polymerase can be used as probes in a wide variety of applications | Cystovirus phi6 |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Cystovirus phi6 |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | Cystovirus phi6 | template is RNA, the phi6 polymerase is highly processive and can use either single- or double-stranded RNA as a template and synthesizes a full-length complementary strand of an RNA | diphosphate + RNAn+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cystovirus phi6 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nucleoside triphosphate + RNAn | template is RNA, the phi6 polymerase is highly processive and can use either single- or double-stranded RNA as a template and synthesizes a full-length complementary strand of an RNA | Cystovirus phi6 | diphosphate + RNAn+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phi6 polymerase | - |
Cystovirus phi6 |
| RDRP | - |
Cystovirus phi6 |
| RNA-dependent RNA polymerase | - |
Cystovirus phi6 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
assay at | Cystovirus phi6 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
assay at | Cystovirus phi6 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | RdRps are unique in that they create dsRNA by initiating polymerization at the 3' end of the substrate | Cystovirus phi6 |
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