Cloned (Comment) | Organism |
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sequence comparisons, expression of mutant enzymes in BHK cells | Lassa mammarenavirus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
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additional information | Lassa mammarenavirus | the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of thedomain, overview | ? | - |
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Organism | UniProt | Comment | Textmining |
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Lassa mammarenavirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of thedomain, overview | Lassa mammarenavirus | ? | - |
? | |
additional information | residues K1127, R1134, E1135, L1136, D1140, and K1144, D1193, G1298, S1333, D1334, D1335, and K1376 from different subunits might be involved in catalysis | Lassa mammarenavirus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the RNA-dependent RNA polymerase of arenaviruses is an integral part of the L protein, a 200-kDa multifunctional and multidomain protein, structure and function of the Lassa virus RdRp domain, folding model of the domain, overview | Lassa mammarenavirus |
Synonyms | Comment | Organism |
---|---|---|
RDRP | - |
Lassa mammarenavirus |
RNA polymerase | - |
Lassa mammarenavirus |
RNA-dependent RNA polymerase | - |
Lassa mammarenavirus |
Organism | Comment | Expression |
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Lassa mammarenavirus | large-scale mutagenesis of the RdRp domain of Lassa virus L protein, overview. Mutation of two adjacent residues in the putative palm-thumb subdomain junction, G1394 and D1395, by PCR-based mutagenesis in strain AV, lead to a defect in mRNA synthesis but do not affect antigenomic RNA synthesis. Clear dominant-negative effects are only seen with mutants affecting residues in motifs pre-A with K1127, R1134, E1135, L1136, D1140, and K1144, A with D1193, B with G1298, C with S1333, D1334, and D1335, and D with K1376, overview | additional information |