Literature summary for 2.7.7.48 extracted from

Ryu, K.; Kim, K.H.; Yoo, S.Y.; Lee, E.Y.; Lim, K.H.; Min, M.K.; Kim, H.; Choi, S.I.; Seong, B.L.
Production and characterization of active hepatitis C virus RNA-dependent RNA polymerase (2010), Protein Expr. Purif., 71, 147-152.

Search for more literature for 2.7.7.48

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli using the N-terminal domain of Escherichia coli lysyl-tRNA synthetase as a solubility enhancer	Hepacivirus C

Inhibitors

Inhibitors	Comment	Organism	Structure
2,4-dioxo-4-phenylbutanoic acid	specific inhibitor	Hepacivirus C
additional information	not inhibitory: rifamycin	Hepacivirus C

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.005	-	UTP	30°C, pH 8.0	Hepacivirus C

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	or Mn2+, required. Assay in presence of 5 mM	Hepacivirus C
Mn2+	or Mg2+, required. Assay in presence of 10 mM	Hepacivirus C

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
82000	-	x * 82000, SDS-PAGE, fusion protein using the N-terminal domain of Escherichia coli lysyl-tRNA synthetase	Hepacivirus C

Organism

Organism	UniProt	Comment	Textmining
Hepacivirus C	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(dT)12 + UTP	assay uses poly(A) as an RNA template and oligo(dT)12-18 as the primer. Enzyme is strictly dependent on the presence of primer	Hepacivirus C	diphosphate + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 82000, SDS-PAGE, fusion protein using the N-terminal domain of Escherichia coli lysyl-tRNA synthetase	Hepacivirus C

Synonyms

Synonyms	Comment	Organism
NS5B	-	Hepacivirus C

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Hepacivirus C

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.004	-	30°C, pH 8.0	Hepacivirus C	2,4-dioxo-4-phenylbutanoic acid

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Release 2023.1 (January 2023)