Application | Comment | Organism |
---|---|---|
pharmacology | the viral RNA polymerase is an attractive target for inhibition in the treatment of viral infections | Dengue virus |
Cloned (Comment) | Organism |
---|---|
bacterial production of the recombinant DENV-2 RdRp was difficult due to its low expression and solubility levels, recombinant expression of three different forms of DENV-2 RdRp as either N- or C-terminally His-tagged fusions, or without tag in Escherichia coli strains Rosetta (DE3) and BL21 (DE3). The presence of both the N- and C-terminal His-tag has a deleterious effect on polymerase activity, overview | Dengue virus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics analysis of non-tagged DENV-2 RdRp in the presence of constant poly(rC) and Mg2+ concentrations | Dengue virus | |
2.39 | - |
GTP | pH 7.5, 30°C, recombinant nontagged enzyme | Dengue virus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Dengue virus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Dengue virus | |
additional information | recombinant non-tagged RdRp does not require manganese ions to activate RNA polymerization | Dengue virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
- |
Dengue virus |
73200 | - |
recombinant nontagged enzyme, gel filtration | Dengue virus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Dengue virus | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dengue virus | P29991 | polyprotein; serotype 2, a flavivirus | - |
Purification (Comment) | Organism |
---|---|
three different recombinant forms of DENV-2 RdRp as either N- or C-terminally His-tagged fusions, or without tag from Escherichia coli strain s Rosetta (DE3) and BL21 (DE3) to homogeneity by nickel affinity and anion exchange chromatography, followed by gel filtration and ultracentrifugation | Dengue virus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + RNAn | with poly(rC) | Dengue virus | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | - |
Dengue virus | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, recombinant nontagged enzyme, SDS-PAGE | Dengue virus |
Synonyms | Comment | Organism |
---|---|---|
RDRP | - |
Dengue virus |
RNA-dependent RNA polymerase | - |
Dengue virus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Dengue virus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.345 | - |
GTP | pH 7.5, 30°C, recombinant nontagged enzyme | Dengue virus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dengue virus |
General Information | Comment | Organism |
---|---|---|
physiological function | the RNA-dependent RNA polymerase activity resides in the C-terminal two-thirds of non-structural protein (NS) 5 responsible for the de novo synthesis of the viral RNA genome. RdRp specifically binds to the viral UTR | Dengue virus |