Cloned (Comment) | Organism |
---|---|
for recombinant expression in Spodoptera frugiperda Sf9 cells via baculovius transfection method, RSV RdRp is split into three pieces, since expression of the full protein cannot be achieved, expression as N-terminally His-tagged proteins or as GFP-fusion proteins | Rice stripe tenuivirus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | analysis of cellular localization in transfected Spodoptera frugiperda Sf9 cells of the three recombinant RdRp fragments and their interactions with nucleocapsid protein, overview. All three RdRp fragments display punctuate staining patterns in the cytoplasm, and the C-terminal fragment colocalizes with the nucleocapsid protein in the perinuclear region, but only the C-terminal fragment can interact with the nucleocapsid protein | Rice stripe tenuivirus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rice stripe tenuivirus | the C-terminal fragment can interact with the viral nucleocapsid protein, the N-terminal 50 amino acids of nucleocapsid protein are responsible for the NP-RdRp interaction | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rice stripe tenuivirus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the C-terminal fragment can interact with the viral nucleocapsid protein, the N-terminal 50 amino acids of nucleocapsid protein are responsible for the NP-RdRp interaction | Rice stripe tenuivirus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RDRP | - |
Rice stripe tenuivirus |
RNA-dependent RNA polymerase | - |
Rice stripe tenuivirus |
RSV RdRp | - |
Rice stripe tenuivirus |
General Information | Comment | Organism |
---|---|---|
physiological function | the RNA-dependent RNA polymerase of rice stripe virus is critical for both the transcription and replication of the viral genome | Rice stripe tenuivirus |