Literature summary for 2.7.7.5 extracted from

Guranowski, A.; Wojdyla, A.; Zimny, J.; Wypijewska, A.; Kowalska, J.; Jemielity, J.; Davis, R.; Bieganowski, P.
Dual activity of certain HIT-proteins: A. thaliana Hint4 and C. elegans DcpS act on adenosine 5'-phosphosulfate as hydrolases (forming AMP) and as phosphorylases (forming ADP) (2010), FEBS Lett., 584, 93-98.

Search for more literature for 2.7.7.5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1	-	adenosine 5'-phosphosulfate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Arabidopsis thaliana
26	-	adenosine 5'-phosphosulfate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Caenorhabditis elegans
46	-	phosphate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Caenorhabditis elegans
600	-	phosphate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q84VV6	-	-
Caenorhabditis elegans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme can act as a sulfohydrolase, catalyzing the liberation of AMP from adenosine 5'-phosphosulfate. However, in the presence of phosphate, the enzyme also behaves as adenosine 5'-phosphosulfate phosphorylases, forming ADP	Arabidopsis thaliana	?	-	?
additional information	the enzyme can act as a sulfohydrolase, catalyzing the liberation of AMP from adenosine 5'-phosphosulfate. However, in the presence of phosphate, the enzyme also behaves as adenosine 5'-phosphosulfate phosphorylases, forming ADP. The enzyme does not use adenosine 5'-phosphoamidate, adenosine 5'-phosphofluoride, and diadenosine tetraphosphate as substrates	Caenorhabditis elegans	?	-	?
additional information	when assayed in 0.1 M KH2PO4 with adenosine 5'-phosphosulfate, the enzyme cannot use diphosphate or triphosphate as potential adenylate acceptors in place of phosphate	Arabidopsis thaliana	?	-	?
additional information	when assayed in 0.1 M KH2PO4 with adenosine 5'-phosphosulfate, the enzyme cannot use diphosphate or tripolyphosphate as potential adenylate acceptors in place of phosphate	Caenorhabditis elegans	?	-	?
phosphate + adenosine 5'-phosphoamidate	22% reaction velocity compared to adenosine 5'-phosphosulfate	Arabidopsis thaliana	ADP + NH4+	-	ir
phosphate + adenosine 5'-phosphofluoride	8% reaction velocity compared to adenosine 5'-phosphosulfate	Arabidopsis thaliana	ADP + fluoride	-	?
phosphate + adenosine 5'-phosphosulfate	100% reaction velocity	Caenorhabditis elegans	ADP + SO42-	-	ir
phosphate + adenosine 5'-phosphosulfate	100% reaction velocity	Arabidopsis thaliana	ADP + SO42-	-	ir
phosphate + diadenosine tetraphosphate	2% reaction velocity compared to adenosine 5'-phosphosulfate	Arabidopsis thaliana	?	-	?
phosphate + diadenosine triphosphate	4% reaction velocity compared to adenosine 5'-phosphosulfate	Caenorhabditis elegans	?	-	?
phosphate + diadenosine triphosphate	7% reaction velocity compared to adenosine 5'-phosphosulfate	Arabidopsis thaliana	?	-	?
phosphate + inosine 5'-phosphosulfate	-	Caenorhabditis elegans	IDP + SO42-	-	ir
phosphate + inosine 5'-phosphosulfate	-	Arabidopsis thaliana	IDP + SO42-	-	ir

Subunits

Subunits	Comment	Organism
homodimer	-	Caenorhabditis elegans
homodimer	-	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
adenosine 5'-phosphosulfate phosphorylase	-	Caenorhabditis elegans
adenosine 5'-phosphosulfate phosphorylase	-	Arabidopsis thaliana
adenylylsulfate:phosphate adenylyltransferase	-	Caenorhabditis elegans
adenylylsulfate:phosphate adenylyltransferase	-	Arabidopsis thaliana
ADP-sulfurylase	misleading	Caenorhabditis elegans
ADP-sulfurylase	misleading	Arabidopsis thaliana
APS phosphorylase	-	Caenorhabditis elegans
APS phosphorylase	-	Arabidopsis thaliana
Dcps	-	Caenorhabditis elegans
Hint4	-	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.6	-	adenosine 5'-phosphosulfate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Caenorhabditis elegans
3	6	adenosine 5'-phosphosulfate	in 0.1 M HEPES/KOH buffer (pH 6.8), 0.1 M potassium phosphate (pH 6.8), 0.1 M KH2PO4 (pH 4.2), at 30°C	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.2	6.8	Hint4 degrades adenosine 5'-phosphosulfate phosphorolytically twice as fast at pH 4.2 than at pH 6.8	Arabidopsis thaliana

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Release 2023.1 (January 2023)