Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.50 extracted from

  • Fresco, L.D.; Buratowski, S.
    Active site of the mRNA-capping enzyme guanylyltransferase from Saccharomyces cerevisiae: similarity to the nucleotidyl attachment motif of DNA and RNA ligases (1994), Proc. Natl. Acad. Sci. USA, 91, 6624-6628.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
gene CEG1, guanylylpeptide, DNA and amino acid sequence determination Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information CEG1 gene products with substitutions at Lys70 are unable to perform the reaction and support the viability in vivo Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + pp(5')RNA Saccharomyces cerevisiae
-
 G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 gene CEG1
-

Reaction

Reaction Comment Organism Reaction ID
GTP + (5')ppPur-mRNA = diphosphate + G(5')pppPur-mRNA active site Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
 Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA reversible formation of a covalent intermediate enzyme-GMP Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r
GTP + pp(5')RNA GMP is linked to the enzyme via a phosphoamide bond to the epsilon-amino group of Lys70 Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue r

Synonyms

Synonyms Comment Organism
mRNA capping enzyme
-
 Saccharomyces cerevisiae