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Literature summary for 2.7.7.60 extracted from

  • Kemp, L.E.; Bond, C.S.; Hunter, W.N.
    Crystallization and preliminary x-ray diffraction studies of recombinant Escherichia coli 4-diphosphocytidyl-2-C-methyl-D-erythritol synthetase (2001), Acta Crystallogr. Sect. D, 57, 1189-1191.
    View publication on PubMed
Search for more literature for 2.7.7.60

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion using paoyethylene glycol as precipitant, elongated tetragonal prismatic crystals of 0.1 * 0.1 * 0.5 mm, space group P41212 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25700
-
 2 * 25700 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli third step of biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal-chelating Hitrap clumn preloaded with Ni2+, Q-Sepharose anion-exchange chromatography to remove previously added thrombin Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + 2-C-methyl-D-erythritol-4-phosphate third step of biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

Subunits Comment Organism
dimer homodimer Escherichia coli
dimer 2 * 25700 Escherichia coli

Synonyms

Synonyms Comment Organism
diphosphocytidyl-methylerythritol synthetase
-
 Escherichia coli