Protein Variants | Comment | Organism |
---|---|---|
H45A | site-directed mutagenesis | Salmonella enterica subsp. enterica serovar Typhimurium |
H45A/H46A | site-directed mutagenesis | Salmonella enterica subsp. enterica serovar Typhimurium |
H46A | site-directed mutagenesis | Salmonella enterica subsp. enterica serovar Typhimurium |
H46N | site-directed mutagenesis | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + adenosylcobinamide phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
diphosphate + adenosylcobinamide-GDP | late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, required for assimilation of exogenous cobinamide and de novo synthesis of adenosylcobalamin, reaction proceeds via a covalently modified CobU-GMP intermediate | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00000002 | - |
GTP:guanylyltransferase specific activity, mutant H46A | Salmonella enterica subsp. enterica serovar Typhimurium |
0.00000003 | - |
GTP:guanylyltransferase specific activity, mutant H46N | Salmonella enterica subsp. enterica serovar Typhimurium |
0.000205 | - |
GTP:guanylyltransferase specific activity, mutant H45A | Salmonella enterica subsp. enterica serovar Typhimurium |
0.00067 | - |
GTP:guanylyltransferase specific activity, wild-type | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + adenosylcobinamide phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + adenosylcobinamide-GDP | - |
? | |
GTP + adenosylcobinamide phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | diphosphate + adenosylcobinamide-GDP | late steps of cobalamin biosynthesis, involved in de novo synthesis of vitamin B12, nucleotide loop assembly, required for assimilation of exogenous cobinamide and de novo synthesis of adenosylcobalamin, reaction proceeds via a covalently modified CobU-GMP intermediate | ? | |
additional information | CobU is a bifunctional enzyme that has both kinase EC 27.1.1.156 and guanyltransferase EC 2.7.7.62 activities | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenosylcobinamide phosphate guanylyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
AdoCbi-P guanylyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |