Application | Comment | Organism |
---|---|---|
synthesis | the thermophilic enzyme is a valuable tool for c-di-GMP synthesis as well as the preparation of c-di-GMP derivatives | Thermotoga maritima |
Cloned (Comment) | Organism |
---|---|
a DNA construct encoding the C-terminus of the TM1788 gene is cloned into the vector pET28b+ for expression in Escherichia col BL21DE3 cells | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
R158A | mutation of a key residue in the putative regulatory I-site, product inhibition is substantially alleviated | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
determined by SDS-PAGE | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | Thermotoga maritima | - |
diphosphate + cyclic di-3',5'-guanylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9X2A8 | - |
- |
Purification (Comment) | Organism |
---|---|
using Ni2+-nitrilotriacetic acid resin and a gel filtration coumn | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP | - |
Thermotoga maritima | diphosphate + cyclic di-3',5'-guanylate | - |
? | |
GTP + GTP | - |
Thermotoga maritima | 2 diphosphate + cyclic di-3',5'-guanylate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | wild-type | Thermotoga maritima |
monomer | R158A mutant | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
DGC | - |
Thermotoga maritima |
diguanylate cyclase | - |
Thermotoga maritima |
tDGC | - |
Thermotoga maritima |
thermophilic diguanylate cyclase domain protein | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
R158A mutant | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0433 | - |
GTP | R158A mutant | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
R158A mutant | Thermotoga maritima |