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Literature summary for 2.7.7.65 extracted from
- An oxygen-sensing diguanylate cyclase and phosphodiesterase couple for c-di-GMP control (2009), Biochemistry, 48, 9764-9774.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| O2 | - |
Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into an Escherichia coli pUC19-based expression vector and into the vector pMAL-c2e | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 18000 | - |
DosCH fragment, codon 2-160, determined by SDS-PAGE | Escherichia coli |
| 50000 | - |
DosC purified from Escherichia coli moderately overexpressing the wild-type dosCP operon, determined by SDS-PAGE | Escherichia coli |
| 95000 | - |
MBP-DosC, determined by SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | Escherichia coli | - |
diphosphate + cyclic di-3',5'-guanylate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AA89 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a Sephadex G25 and a DEAE-Sepharose column, furthermore an amylose-resin matrix is used | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP | - |
Escherichia coli | diphosphate + cyclic di-3',5'-guanylate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | DosCH fragment | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DGC | - |
Escherichia coli |
| diguanylate cyclase | - |
Escherichia coli |
| direct oxygen sensing cyclase | - |
Escherichia coli |
| DosC | - |
Escherichia coli |
| EcDosC | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
activity assay | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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