Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Kornberg, T.; Kornberg, A.
    Bacterial DNA polymerases (1974), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 10, 119-144.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ara-CTP no inhibition of pol I and III Bacillus subtilis
Ara-CTP pol II Escherichia coli
hydroxyphenylazouracil inhibits only polymerase III Bacillus subtilis
additional information
-
Bacillus subtilis
additional information
-
Bacteria
additional information pol II and III not inhibited by pol I antiserum Escherichia coli
additional information
-
Micrococcus luteus
SH-blocking agents pol II and III Bacillus subtilis
SH-blocking agents pol II and III Bacteria
SH-blocking agents pol II and III Escherichia coli
SH-blocking agents pol II and III Micrococcus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
109000
-
pol I Escherichia coli
110000
-
polymerase I Micrococcus luteus
115000
-
polymerase I Bacillus subtilis
160000
-
pol III Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Bacteria
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Micrococcus luteus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Bacillus subtilis physiological role of pol I and pol III diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Escherichia coli physiological role of pol I, II and pol III diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacteria
-
-
-
Escherichia coli
-
pol II
-
Escherichia coli
-
pol I
-
Escherichia coli
-
pol III
-
Micrococcus luteus
-
synonym: Micrococcus lysodeikticus
-

Reaction

Reaction Comment Organism Reaction ID
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Bacteria
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Bacillus subtilis
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Escherichia coli
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 mechanism Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Bacteria diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol III Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Bacteria diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn no exonuclease 5'--3' activity: pol II Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol II Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn can initiate polymer synthesis de novo, pol I Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn single strands, pol I, but not pol II and III Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn single strands, pol I, but not pol II and III Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Bacteria diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol I Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity, pol I Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of polymerase I Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of polymerase I Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex is no substrate of pol II and III of E. coli Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Bacteria diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn nicked duplex, as poly d(A-T), pol I Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn can not initiate polymer synthesis de novo: pol II and III Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity, pol I Escherichia coli diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity, pol I Micrococcus luteus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn physiological role of pol I and pol III Bacillus subtilis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn physiological role of pol I, II and pol III Escherichia coli diphosphate + DNAn+1
-
?
nucleotide + DNAn
-
Escherichia coli ?
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
pol I and II stable, pol III not Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.17
-
Nucleotide pol II Escherichia coli
16.7
-
Nucleotide pol I Escherichia coli
250
-
Nucleotide pol III Escherichia coli