Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ara-CTP | no inhibition of pol I and III | Bacillus subtilis | |
Ara-CTP | pol II | Escherichia coli | |
hydroxyphenylazouracil | inhibits only polymerase III | Bacillus subtilis | |
additional information | - |
Bacillus subtilis | |
additional information | - |
Bacteria | |
additional information | pol II and III not inhibited by pol I antiserum | Escherichia coli | |
additional information | - |
Micrococcus luteus | |
SH-blocking agents | pol II and III | Bacillus subtilis | |
SH-blocking agents | pol II and III | Bacteria | |
SH-blocking agents | pol II and III | Escherichia coli | |
SH-blocking agents | pol II and III | Micrococcus luteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
109000 | - |
pol I | Escherichia coli |
110000 | - |
polymerase I | Micrococcus luteus |
115000 | - |
polymerase I | Bacillus subtilis |
160000 | - |
pol III | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Bacteria | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Micrococcus luteus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Bacillus subtilis | physiological role of pol I and pol III | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Escherichia coli | physiological role of pol I, II and pol III | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacteria | - |
- |
- |
Escherichia coli | - |
pol II | - |
Escherichia coli | - |
pol I | - |
Escherichia coli | - |
pol III | - |
Micrococcus luteus | - |
synonym: Micrococcus lysodeikticus | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Bacteria | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Bacillus subtilis | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Escherichia coli | |
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 | mechanism | Micrococcus luteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Bacteria | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity, pol III | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 5'--3' activity: pol II | Bacteria | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 5'--3' activity: pol II | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 5'--3' activity: pol II | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 5'--3' activity: pol II | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity, pol II | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | can initiate polymer synthesis de novo, pol I | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | can initiate polymer synthesis de novo, pol I | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | can initiate polymer synthesis de novo, pol I | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | single strands, pol I, but not pol II and III | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | single strands, pol I, but not pol II and III | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | single-stranded 5'-ends greater than 100 nucleotides, pol I, but not pol II and pol III | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Bacteria | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time, cannot initiate a chain de novo, requires a primer which may be DNA or RNA | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity, pol I | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 3'--5' activity, pol I | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex is no substrate of polymerase I | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex is no substrate of polymerase I | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex is no substrate of pol II and III of E. coli | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex, as poly d(A-T), pol I | Bacteria | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex, as poly d(A-T), pol I | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex, as poly d(A-T), pol I | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | nicked duplex, as poly d(A-T), pol I | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | can not initiate polymer synthesis de novo: pol II and III | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity, pol I | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity, pol I | Micrococcus luteus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | physiological role of pol I and pol III | Bacillus subtilis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | physiological role of pol I, II and pol III | Escherichia coli | diphosphate + DNAn+1 | - |
? | |
nucleotide + DNAn | - |
Escherichia coli | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
pol I and II stable, pol III not | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.17 | - |
Nucleotide | pol II | Escherichia coli | |
16.7 | - |
Nucleotide | pol I | Escherichia coli | |
250 | - |
Nucleotide | pol III | Escherichia coli |