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Literature summary for 2.7.7.7 extracted from

  • Hamilton, L.D.G.
    The purification of the DNA polymerase from Micrococcus luteus (1974), Methods Enzymol., 29, 38-45.
    View publication on PubMed
Search for more literature for 2.7.7.7

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, Mg2+ or Mn2+ Micrococcus luteus
Mg2+ stimulates at 4 mM Micrococcus luteus
Mn2+ stimulates at 0.2 mM Micrococcus luteus
Mn2+ divalent cation required, Mn2+ or Mg2+ Micrococcus luteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Micrococcus luteus
-
 diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Micrococcus luteus
-
 synonym: Micrococcus lysodeikticus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Micrococcus luteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.66 1.66
-
 Micrococcus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
 Micrococcus luteus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn exonuclease 5'--3' activity Micrococcus luteus diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn exonuclease 3'--5' activity Micrococcus luteus diphosphate + DNAn+1
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3 7.5 potassium phosphate buffer Micrococcus luteus
7.5 8 Tris-HCl buffer Micrococcus luteus