Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carbonyldiphosphonate | 50% inhibition at 0.15 mM | Leishmania mexicana | |
additional information | no inhibition by 2',3'-dideoxythymidine 5'-triphosphate; no inhibition by aphidicolin; no inhibition by ara-CTP | Leishmania mexicana | |
N-ethylmaleimide | 50% inhibition at less than 1 mM | Leishmania mexicana | |
Salt | sensitive to high ionic strength | Leishmania mexicana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration: 8 mM | Leishmania mexicana | |
Mn2+ | can partially replace Mg2+ in activation | Leishmania mexicana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
145000 | - |
gel filtration | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Leishmania mexicana | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DNA polymerase A | Leishmania mexicana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00019 | - |
polymerase A | Leishmania mexicana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Leishmania mexicana | diphosphate + DNAn+1 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, less than 10% of initial activity, at high and low protein concentration | Leishmania mexicana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania mexicana | - |
- |
7 |