Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Sellmann, E.; Schröder, K.L.; Knoblich, I.M.; Westermann, P.
    Purification and characterization of DNA polymerases from Bacillus species (1992), J. Bacteriol., 174, 4350-4355.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology thermostable polymerase used in PCR Thermus thermophilus
molecular biology thermostable polymerase used in PCR Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
Dideoxynucleoside 5'-triphosphate
-
[Bacillus] caldotenax
Dideoxynucleoside 5'-triphosphate
-
[Bacillus] caldovelox
Dideoxynucleoside 5'-triphosphate
-
Geobacillus stearothermophilus
Dideoxynucleoside 5'-triphosphate
-
Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulates Thermus thermophilus
K+ stimulates Geobacillus stearothermophilus
K+ stimulates [Bacillus] caldotenax
K+ stimulates [Bacillus] caldovelox
K+ 5-100 mM Thermus thermophilus
K+ stimulates at 100-200 mM Thermus thermophilus
K+ stimulates at 100-200 mM Geobacillus stearothermophilus
K+ stimulates at 100-200 mM [Bacillus] caldovelox
Mg2+ divalent cation required, Mg2+ or Mn2+ Thermus thermophilus
Mg2+ divalent cation required, Mg2+ or Mn2+ Geobacillus stearothermophilus
Mg2+ divalent cation required, Mg2+ or Mn2+ [Bacillus] caldotenax
Mg2+ divalent cation required, Mg2+ or Mn2+ [Bacillus] caldovelox
Mg2+ optimal concentration: 5-30 mM Geobacillus stearothermophilus
Mg2+ optimal concentration: 5-30 mM [Bacillus] caldotenax
Mg2+ optimal concentration: 5-30 mM [Bacillus] caldovelox
Mg2+ optimal concentration: 20-30 mM Thermus thermophilus
Mn2+ stimulates at 0.4-0.8 mM Thermus thermophilus
Mn2+ stimulates at 0.4 mM Thermus thermophilus
Mn2+ stimulates at 0.4 mM Geobacillus stearothermophilus
Mn2+ stimulates at 0.4 mM [Bacillus] caldovelox
Mn2+ divalent cation required, Mn2+ or Mg2+ Thermus thermophilus
Mn2+ divalent cation required, Mn2+ or Mg2+ Geobacillus stearothermophilus
Mn2+ divalent cation required, Mn2+ or Mg2+ [Bacillus] caldotenax
Mn2+ divalent cation required, Mn2+ or Mg2+ [Bacillus] caldovelox

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
SDS-PAGE Thermus thermophilus
95000
-
SDS-PAGE Geobacillus stearothermophilus
95000
-
SDS-PAGE [Bacillus] caldovelox

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Thermus thermophilus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Geobacillus stearothermophilus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn [Bacillus] caldotenax
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn [Bacillus] caldovelox
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Thermus thermophilus
-
-
-
[Bacillus] caldotenax
-
-
-
[Bacillus] caldovelox
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus
-
Geobacillus stearothermophilus
-
[Bacillus] caldotenax
-
[Bacillus] caldovelox

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
Thermus thermophilus
20.8
-
-
[Bacillus] caldovelox
26.7
-
-
Geobacillus stearothermophilus
33.3
-
-
[Bacillus] caldotenax

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermus thermophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Geobacillus stearothermophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
[Bacillus] caldotenax diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
[Bacillus] caldovelox diphosphate + DNAn+1
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 65
-
Geobacillus stearothermophilus
65 70
-
[Bacillus] caldotenax
65 70
-
[Bacillus] caldovelox
70 75
-
Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
10 min, inactivation Geobacillus stearothermophilus
85
-
10 min, inactivation [Bacillus] caldotenax
85
-
10 min, inactivation [Bacillus] caldovelox
95
-
10 min, inactivation Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
-
Thermus thermophilus
8.8
-
-
Geobacillus stearothermophilus
8.8
-
-
[Bacillus] caldotenax
8.8
-
-
[Bacillus] caldovelox