Application | Comment | Organism |
---|---|---|
biotechnology | thermostable polymerase used in PCR | Thermus thermophilus |
molecular biology | thermostable polymerase used in PCR | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dideoxynucleoside 5'-triphosphate | - |
[Bacillus] caldotenax | |
Dideoxynucleoside 5'-triphosphate | - |
[Bacillus] caldovelox | |
Dideoxynucleoside 5'-triphosphate | - |
Geobacillus stearothermophilus | |
Dideoxynucleoside 5'-triphosphate | - |
Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stimulates | Thermus thermophilus | |
K+ | stimulates | Geobacillus stearothermophilus | |
K+ | stimulates | [Bacillus] caldotenax | |
K+ | stimulates | [Bacillus] caldovelox | |
K+ | 5-100 mM | Thermus thermophilus | |
K+ | stimulates at 100-200 mM | Thermus thermophilus | |
K+ | stimulates at 100-200 mM | Geobacillus stearothermophilus | |
K+ | stimulates at 100-200 mM | [Bacillus] caldovelox | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Thermus thermophilus | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | Geobacillus stearothermophilus | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | [Bacillus] caldotenax | |
Mg2+ | divalent cation required, Mg2+ or Mn2+ | [Bacillus] caldovelox | |
Mg2+ | optimal concentration: 5-30 mM | Geobacillus stearothermophilus | |
Mg2+ | optimal concentration: 5-30 mM | [Bacillus] caldotenax | |
Mg2+ | optimal concentration: 5-30 mM | [Bacillus] caldovelox | |
Mg2+ | optimal concentration: 20-30 mM | Thermus thermophilus | |
Mn2+ | stimulates at 0.4-0.8 mM | Thermus thermophilus | |
Mn2+ | stimulates at 0.4 mM | Thermus thermophilus | |
Mn2+ | stimulates at 0.4 mM | Geobacillus stearothermophilus | |
Mn2+ | stimulates at 0.4 mM | [Bacillus] caldovelox | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Thermus thermophilus | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | Geobacillus stearothermophilus | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | [Bacillus] caldotenax | |
Mn2+ | divalent cation required, Mn2+ or Mg2+ | [Bacillus] caldovelox |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
SDS-PAGE | Thermus thermophilus |
95000 | - |
SDS-PAGE | Geobacillus stearothermophilus |
95000 | - |
SDS-PAGE | [Bacillus] caldovelox |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermus thermophilus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Geobacillus stearothermophilus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | [Bacillus] caldotenax | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | [Bacillus] caldovelox | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
[Bacillus] caldotenax | - |
- |
- |
[Bacillus] caldovelox | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
- |
Geobacillus stearothermophilus |
- |
[Bacillus] caldotenax |
- |
[Bacillus] caldovelox |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Thermus thermophilus |
20.8 | - |
- |
[Bacillus] caldovelox |
26.7 | - |
- |
Geobacillus stearothermophilus |
33.3 | - |
- |
[Bacillus] caldotenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermus thermophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Geobacillus stearothermophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
[Bacillus] caldotenax | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
[Bacillus] caldovelox | diphosphate + DNAn+1 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 65 | - |
Geobacillus stearothermophilus |
65 | 70 | - |
[Bacillus] caldotenax |
65 | 70 | - |
[Bacillus] caldovelox |
70 | 75 | - |
Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
10 min, inactivation | Geobacillus stearothermophilus |
85 | - |
10 min, inactivation | [Bacillus] caldotenax |
85 | - |
10 min, inactivation | [Bacillus] caldovelox |
95 | - |
10 min, inactivation | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Thermus thermophilus |
8.8 | - |
- |
Geobacillus stearothermophilus |
8.8 | - |
- |
[Bacillus] caldotenax |
8.8 | - |
- |
[Bacillus] caldovelox |