Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Biles, B.D.; Connolly, B.A.
    Low-fidelity Pyrococcus furiosus DNA polymerase mutants useful in error-prone PCR (2004), Nucleic Acids Res., 32, e176.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D473G wild-type variant Pfu-Pol(exo-) is is 60fold less accurate than Pfu-Pol(exo+) Pyrococcus furiosus
T471A less accurate, by factors of 1.6 than the wild-type variant Pfu-Pol(exo-) Pyrococcus furiosus
T471G less accurate, by factors of 1.2 than the wild-type variant Pfu-Pol(exo-) Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn wild-type Pfu-Pol makes about one mistake for every 1000000 bases incorporated, wild-type variant Pfu-Pol(exo-)(D473F)is 60fold less accurate Pyrococcus furiosus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Pfu-POl
-
Pyrococcus furiosus