Organism | UniProt | Comment | Textmining |
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Saccharolobus solfataricus | - |
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- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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deoxynucleoside triphosphate + DNAn | with template guanine and Watson-Crick paired dCTP as the nascent base pair. Water-mediated and substrate-assisted mechanism: the initial proton transfer to the R-phosphate of the substrate via a bridging crystal water molecule is the rate-limiting step, the nucleotidyl-transfer step is associative with a metastable pentacovalent phosphorane intermediate, and the diphosphate leaving is facilitated by a highly coordinated proton relay mechanism through mediation of water which neutralizes the evolving negative charge. The conserved carboxylates, which retain their liganding to the two Mg2+ ions during the reaction process, are found to be essential in stabilizing transition states. This water-mediated and substrate-assisted mechanism takes specific advantage of the unique structural features of this low-fidelity lesion-bypass Y-family polymerase, which has a more spacious and solvent-exposed active site than replicative and repair polymerases | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
additional information | lesion-bypass DNA polymerase | Saccharolobus solfataricus | ? | - |
? |
Synonyms | Comment | Organism |
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DNA polymerase IV | - |
Saccharolobus solfataricus |