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Literature summary for 2.7.7.7 extracted from

  • Takeuchi, R.; Kimura, S.; Saotome, A.; Sakaguchi, K.
    Biochemical properties of a plastidial DNA polymerase of rice (2007), Plant Mol. Biol., 64, 601-611.
    View publication on PubMed
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
-
 Oryza sativa

Protein Variants

Protein Variants Comment Organism
D362A the misincorporation frequency values of the D362A mutant are slightly higher (1.4–4.9fold) than those of the wild type protein Oryza sativa
additional information POLPs have two extra sequences in the polymerase domain that are absent in prokaryotic PolIs. Deletion of either insert from OsPOLP1 causes a decrease in DNA synthetic activity, processivity, and DNA binding activity Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00002772
-
 dCMP:dG 37°C, pH 7.5, wild-type enzyme, substitution opposite the template G Oryza sativa
0.00002847
-
 dCMP:dG 37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G Oryza sativa
0.00003176
-
 dTMP:dA 37°C, pH 7.5, wild-type enzyme, substitution opposite the template A Oryza sativa
0.1056
-
 dAMP:dA 37°C, pH 7.5, wild-type enzyme, substitution opposite the template A Oryza sativa
0.263
-
 dGMP:dG 37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G Oryza sativa
0.3511
-
 dGMP:dG 37°C, pH 7.5, wild-type enzyme, substitution opposite the template G Oryza sativa
0.551
-
 dCMP:dA 37°C, pH 7.5, wild-type enzyme, substitution opposite the template A Oryza sativa
1.153
-
 dAMP:dG 37°C, pH 7.5, wild-type enzyme, substitution opposite the template G Oryza sativa
1.26
-
 dTMP:dG 37°C, pH 7.5, wild-type enzyme, substitution opposite the template G Oryza sativa
1.42
-
 dAMP:dG 37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G Oryza sativa
1.43
-
 dTMP:dG 37°C, pH 7.5, mutant enzyme D362A, substitution opposite the template G Oryza sativa
2.091
-
 dGMP:dA 37°C, pH 7.5, wild-type enzyme, substitution opposite the template A Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Oryza sativa 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Oryza sativa OsPOLP1 might be involved in a repair pathway similar to long-patch base excision repair. Possible role of POLPs in plastidial DNA replication and repair diphosphate + DNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST fusion protein containing OsPOLP1 Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn OsPOLP1 might be involved in a repair pathway similar to long-patch base excision repair. Possible role of POLPs in plastidial DNA replication and repair Oryza sativa diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn OsPOLP1 efficiently catalyzed strand displacement on nicked DNA with a 5'-deoxyribose phosphate Oryza sativa diphosphate + DNAn+1
-
 ?
dNTP + dAMP:dA
-
 Oryza sativa ?
-
 ?
dNTP + dAMP:dG
-
 Oryza sativa ?
-
 ?
dNTP + dCMP:dA
-
 Oryza sativa ?
-
 ?
dNTP + dCMP:dG
-
 Oryza sativa ?
-
 ?
dNTP + dGMP:dA
-
 Oryza sativa ?
-
 ?
dNTP + dGMP:dG
-
 Oryza sativa ?
-
 ?
dNTP + dTMP:dA
-
 Oryza sativa ?
-
 ?
dNTP + dTMP:dG
-
 Oryza sativa ?
-
 ?

Synonyms

Synonyms Comment Organism
OsPOLP1
-
 Oryza sativa