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Literature summary for 2.7.7.7 extracted from

  • Ketkar, A.; Zafar, M.K.; Banerjee, S.; Marquez, V.E.; Egli, M.; Eoff, R.L.
    Differential furanose selection in the active sites of archaeal DNA polymerases probed by fixed-conformation nucleotide analogues (2012), Biochemistry, 51, 9234-9244.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of Dpo4 in complex with North-methanocarba-dATP opposite dT Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.0013
-
South-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.0014
-
dATP pH 7.5, 37°C Saccharolobus solfataricus
0.0087
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.01
-
dATP pH 7.5, 37°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus Q97W02
-
-
Saccharolobus solfataricus P2
-
-
-
Saccharolobus solfataricus P2 Q97W02
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dATP + DNAn
-
Saccharolobus solfataricus ?
-
?
dATP + DNAn
-
Saccharolobus solfataricus P2 ?
-
?
North-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme preferentially inserts North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker compared to a South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus ?
-
?
North-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus ?
-
?
North-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus P2 ?
-
?
North-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme preferentially inserts North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker compared to a South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus P2 ?
-
?
South-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus ?
-
?
South-methanocarba-dATP + DNAn the role of sugar geometry during nucleotide selection is probed by the enzyme from Sulfolobus solfataricus using fixed conformation nucleotide analogues. The enzyme relatively tolerant to the substrate conformation: North-methanocarba-dATP that locks the central ring into a RNA-type (C2'-exo, North) conformation near a C3'-endo pucker or South-methanocarba-dATP that locks the central ring system into a (C3'-exo, South) conformation near a C2'-endo pucker Saccharolobus solfataricus P2 ?
-
?

Synonyms

Synonyms Comment Organism
Dpo1
-
Saccharolobus solfataricus
Dpo4
-
Saccharolobus solfataricus
SSO2448 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0088
-
South-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.014
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.023
-
dATP pH 7.5, 37°C Saccharolobus solfataricus
0.08
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
0.3
-
dATP pH 7.5, 37°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.8
-
South-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
9.2
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
11.7
-
North-methanocarba-dATP pH 7.5, 37°C Saccharolobus solfataricus
16.43
-
dATP pH 7.5, 37°C Saccharolobus solfataricus
30
-
dATP pH 7.5, 37°C Saccharolobus solfataricus