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Literature summary for 2.7.7.7 extracted from

  • McDonald, J.P.; Hall, A.; Gasparutto, D.; Cadet, J.; Ballantyne, J.; Woodgate, R.
    Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs (2006), Nucleic Acids Res., 34, 1102-1111.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications Acidianus infernus
analysis the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications Sulfurisphaera ohwakuensis
analysis the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications Sulfolobus tengchongensis
analysis the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications Saccharolobus shibatae
analysis the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications Stygiolobus azoricus

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) Acidianus infernus
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) Sulfurisphaera ohwakuensis
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) Sulfolobus tengchongensis
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) Saccharolobus shibatae
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) Stygiolobus azoricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Acidianus infernus
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Sulfurisphaera ohwakuensis
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Sulfolobus tengchongensis
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Saccharolobus shibatae
-
diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn Stygiolobus azoricus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Acidianus infernus Q2FA65
-
-
Saccharolobus shibatae Q2FA62
-
-
Stygiolobus azoricus Q2FA64
-
-
Sulfolobus tengchongensis Q2FA66
-
-
Sulfurisphaera ohwakuensis Q2FA63
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acidianus infernus
-
Sulfurisphaera ohwakuensis
-
Sulfolobus tengchongensis
-
Saccharolobus shibatae
-
Stygiolobus azoricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Acidianus infernus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Sulfurisphaera ohwakuensis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Sulfolobus tengchongensis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Saccharolobus shibatae diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Stygiolobus azoricus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq Acidianus infernus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq Sulfurisphaera ohwakuensis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq Sulfolobus tengchongensis diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq Saccharolobus shibatae diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq Stygiolobus azoricus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
Dpo4-like enzyme
-
Acidianus infernus
Dpo4-like enzyme
-
Sulfurisphaera ohwakuensis
Dpo4-like enzyme
-
Sulfolobus tengchongensis
Dpo4-like enzyme
-
Saccharolobus shibatae
Dpo4-like enzyme
-
Stygiolobus azoricus