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Literature summary for 2.7.7.8 extracted from

  • Hishinuma, F.; Hirai, K.; Sakaguchi, K.
    Thermophilic polynucleotide phosphorylase from Thermus thermophilus. Purification and properties of an altered form of enzyme which lacks phosphorolytic activity to polynucleotide (1977), Eur. J. Biochem., 77, 575-583.
    View publication on PubMed

General Stability

General Stability Organism
stable against several freezing and thawing cycles Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE Thermus thermophilus
73000
-
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE Thermus thermophilus
92000
-
1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE Thermus thermophilus
190000
-
native PAGE, gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
HB-8 strain
-

Purification (Commentary)

Purification (Comment) Organism
ultracentrifugation, ammonium sulfate, QAE-Sephadex a-25, DEAE-cellulose, hydroxyapatite, purified enzyme loses poly(A) phosphorylating activity Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1
-
-
Thermus thermophilus

Storage Stability

Storage Stability Organism
-20°C, dilute solution, at least 2 months, no loss of activity Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(A) + ADP primer required for polymerization Thermus thermophilus poly(A)+1 + phosphate
-
r
poly(C) + CDP primer required for polymerization Thermus thermophilus poly(C)+1 + phosphate
-
r
poly(G) + GDP primer required for polymerization Thermus thermophilus poly(G)+1 + phosphate
-
r
poly(I) + IDP primer required for polymerization Thermus thermophilus poly(I)+1 + phosphate
-
r
poly(U)+ UDP primer required for polymerization Thermus thermophilus poly(U)+1 + phosphate
-
r
polyadenylic acid + phosphate
-
Thermus thermophilus ? + ADP
-
?
polycytidylic acid + phosphate
-
Thermus thermophilus ? + CDP
-
?
polyguanylic acid + phosphate
-
Thermus thermophilus ? + GDP
-
?
polyuridylic acid + phosphate
-
Thermus thermophilus ? + UDP
-
?
RNAn + a nucleoside diphosphate primer-dependent activity Thermus thermophilus RNAn+1 + phosphate
-
?
RNAn + a nucleoside diphosphate ADP, GDP, UDP and CDP polymerized to the extent of 7 S size polymer Thermus thermophilus RNAn+1 + phosphate
-
?

Subunits

Subunits Comment Organism
trimer 1 * 92000 + 1 * 73000 + 1 * 35000, SDS-PAGE Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
polymerization of UDP with (Ap)3A as primer Thermus thermophilus
70
-
poymerization of ADP, CDP or GDP with (Ap)3A as primer Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
10 min, 0.05 mg/ml enzyme, less than 10% loss of activity Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.33
-
Polyadenylic acid synthesis of a copolymer of adenylic acid Thermus thermophilus
3.33
-
polyguanylic acid synthesis of a copolymer of guanylic acid Thermus thermophilus
3.33
-
polyuridylic acid synthesis of a copolymer of uridylic acid Thermus thermophilus
3.33
-
Polycytidylic acid synthesis of a copolymer of cytidylic acid Thermus thermophilus
75
-
Polyadenylic acid
-
Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.7
-
poymerization of ADP, CDP or UDP Thermus thermophilus
10.5
-
polymerization of GDP Thermus thermophilus