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Literature summary for 2.7.7.9 extracted from
- Sugar nucleotide inhibition of UDP-glucose pyrophosphorylase from calf liver (1973), Biochim. Biophys. Acta, 309, 307-317.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-galactose 1-phosphate | no inhibition | Bos taurus |  |
| additional information | inhibition kinetics for UDP-sugars; little or no inhibition by alpha-D-mannose 1-phosphate, alpha-D-xylose 1-phosphate or alpha-D-glucuronic acid 1-phosphate | Bos taurus | |
| UDP-D-mannose | - |
Bos taurus | |
| UDP-galactose | 14% inhibition at 0.35 mM | Bos taurus | |
| UDP-galacturonic acid | 48% inhibition at 0.43 m | Bos taurus | |
| UDP-glucose | 87% inhibition at 0.08 mM | Bos taurus | |
| UDP-glucuronic acid | 56% inhibition at 0.45 mM | Bos taurus | |
| UDP-mannose | 23% inhibition at 0.45 mM | Bos taurus | |
| UDP-xylose | 32% inhibition at 0.36 mM | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | 0.048 | alpha-D-glucose 1-phosphate | pH 7.5, 30°C | Bos taurus | |
| 0.1 | 0.2 | UTP | pH 7.5, 30°C | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activation | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
male calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.95 | - |
partially purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Bos taurus | diphosphate + UDP-glucose | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
UDP-D-glucose | pH 7.5, 30°C | Bos taurus | |
| 0.15 | - |
UDP-D-glucuronic acid | pH 7.5, 30°C | Bos taurus | |
| 0.21 | - |
UDP-D-galacturonic acid | pH 7.5, 30°C | Bos taurus | |
| 0.24 | - |
UDP-D-xylose | pH 7.5, 30°C | Bos taurus | |
| 4.8 | - |
UDP-D-galactose | pH 7.5, 30°C | Bos taurus | |
| 9.6 | - |
UDP-D-mannose | pH 7.5, 30°C | Bos taurus | |
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Release 2023.1 (January 2023)
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