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Literature summary for 2.7.7.9 extracted from
- Uridine diphosphate glucose pyrophosphorylase. Crystallization and properties of the enzyme from rabbit liver and species comparisons (1974), J. Biol. Chem., 249, 7695-7700.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bos taurus |  |
| 2-mercaptoethanol | requirement | Homo sapiens | |
| 2-mercaptoethanol | no activation | Oryctolagus cuniculus | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| from ammonium sulfate precipitate | Bos taurus |
| from ammonium sulfate precipitate | Oryctolagus cuniculus |
General Stability
| General Stability | Organism |
|---|---|
| enzyme requires 2-mercaptoethanol for stability | Homo sapiens |
| enzyme requires 2-mercaptoethanol for stability | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-galactose 1-phosphate | - |
Bos taurus | |
| alpha-D-galactose 1-phosphate | competitive | Oryctolagus cuniculus | |
| UDP-galactose | - |
Bos taurus | |
| UDP-galactose | - |
Homo sapiens | |
| UDP-galactose | competitive | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | 0.048 | alpha-D-glucose 1-phosphate | - |
Bos taurus | |
| 0.03 | 0.048 | alpha-D-glucose 1-phosphate | pH 7.8 | Oryctolagus cuniculus | |
| 0.048 | - |
UTP | pH 7.8 | Oryctolagus cuniculus | |
| 0.048 | - |
UTP | liver | Homo sapiens | |
| 0.05 | 0.066 | UDP-glucose | - |
Homo sapiens | |
| 0.05 | 0.066 | UDP-glucose | - |
Bos taurus | |
| 0.05 | 0.066 | UDP-glucose | pH 7.8 | Oryctolagus cuniculus | |
| 0.055 | 0.08 | alpha-D-glucose 1-phosphate | - |
Bos taurus | |
| 0.055 | 0.08 | alpha-D-glucose 1-phosphate | pH 7.8 | Oryctolagus cuniculus | |
| 0.084 | - |
diphosphate | - |
Bos taurus | |
| 0.084 | - |
diphosphate | pH 7.8 | Oryctolagus cuniculus | |
| 0.095 | - |
alpha-D-glucose 1-phosphate | - |
Homo sapiens | |
| 0.095 | - |
alpha-D-glucose 1-phosphate | pH 7.8 | Oryctolagus cuniculus | |
| 0.21 | - |
diphosphate | - |
Homo sapiens | |
| 0.21 | - |
diphosphate | pH 7.8 | Oryctolagus cuniculus | |
| 0.42 | - |
UDP-galactose | pH 7.8 | Oryctolagus cuniculus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation | Homo sapiens | |
| Ca2+ | activation | Bos taurus | |
| Ca2+ | activation | Oryctolagus cuniculus | |
| Ca2+ | 28% as effective as Mg2+ at 1 mM | Bos taurus | |
| Ca2+ | 16% as effective as Mg2+ at 2 mM | Homo sapiens | |
| Ca2+ | 50% as effective as Mg2+ at 1 mM | Oryctolagus cuniculus | |
| Co2+ | activation | Homo sapiens | |
| Co2+ | activation | Bos taurus | |
| Co2+ | activation | Oryctolagus cuniculus | |
| Co2+ | 75% activity at 2 mM | Bos taurus | |
| Co2+ | 47-49% activity at 2 mM | Homo sapiens | |
| Co2+ | 47-49% activity at 2 mM | Oryctolagus cuniculus | |
| Mg2+ | requirement | Homo sapiens | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | requirement | Oryctolagus cuniculus | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | maximal activity at 1 mM | Bos taurus | |
| Mn2+ | maximal activity at 1 mM | Oryctolagus cuniculus | |
| additional information | requirement for divalent cations | Oryctolagus cuniculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
8 * 50000, SDS-PAGE, stacked tetrameric configuration | Oryctolagus cuniculus |
| 56000 | - |
8 * 56000, SDS-PAGE | Homo sapiens |
| 60000 | - |
8 * 60000, SDS-PAGE | Bos taurus |
| 400000 | - |
sucrose density gradient centrifugation | Oryctolagus cuniculus |
| 450000 | - |
sucrose density gradient centrifugation | Homo sapiens |
| 480000 | - |
sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
crystallized liver enzyme | - |
| Homo sapiens | - |
crystallized liver enzyme | - |
| Oryctolagus cuniculus | - |
crystallized liver enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from liver | Oryctolagus cuniculus |
| preparative sucrose density gradient centrifugation | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Bos taurus | - |
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 200 | - |
- |
Homo sapiens |
| 200 | - |
- |
Bos taurus |
| 200 | - |
purified enzyme | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + TDP-glucose | poor substrate | Homo sapiens | TTP + alpha-D-glucose 1-phosphate | - |
r | |
| diphosphate + TDP-glucose | poor substrate | Bos taurus | TTP + alpha-D-glucose 1-phosphate | - |
r | |
| diphosphate + TDP-glucose | poor substrate | Oryctolagus cuniculus | TTP + alpha-D-glucose 1-phosphate | - |
r | |
| diphosphate + UDP-xylose | poor substrate | Homo sapiens | UTP + D-xylose 1-phosphate | - |
r | |
| diphosphate + UDP-xylose | poor substrate | Bos taurus | UTP + D-xylose 1-phosphate | - |
r | |
| diphosphate + UDP-xylose | poor substrate | Oryctolagus cuniculus | UTP + D-xylose 1-phosphate | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | about 15% of the activity with UDP-glucose | Bos taurus | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | equilibrium dialysis: 8 mol UDP-galactose bound per mol enzyme | Oryctolagus cuniculus | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | 3.5% of the activity with UDP-glucose | Oryctolagus cuniculus | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-galactose 1-phosphate | 1-12% of the activity with UDP-glucose | Homo sapiens | diphosphate + UDP-galactose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Homo sapiens | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | - |
Bos taurus | diphosphate + UDP-glucose | - |
r | |
| UTP + alpha-D-glucose 1-phosphate | equilibrium dialysis: 8 mol UDP-glucose bound per mol enzyme | Oryctolagus cuniculus | diphosphate + UDP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 50000, SDS-PAGE, stacked tetrameric configuration | Oryctolagus cuniculus |
| octamer | 8 * 56000, SDS-PAGE | Homo sapiens |
| octamer | 8 * 60000, SDS-PAGE | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.5 | broad | Bos taurus |
| 7 | 10.5 | broad | Oryctolagus cuniculus |
| 7.6 | 9.2 | broad | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | - |
Bos taurus | |
| additional information | - |
additional information | - |
Oryctolagus cuniculus |
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