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Literature summary for 2.7.7.9 extracted from

  • Lamerz, A.C.; Haselhorst, T.; Bergfeld, A.K.; von Itzstein, M.; Gerardy-Schahn, R.
    Molecular cloning of the Leishmania major UDP-glucose pyrophosphorylase, functional characterization, and ligand binding analyses using NMR spectroscopy (2006), J. Biol. Chem., 281, 16314-16322.
    View publication on PubMed
Search for more literature for 2.7.7.9

Cloned(Commentary)

Cloned (Comment) Organism
-
 Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 UTP pH 7.8, 25°C Leishmania major
0.105
-
 UDP-glucose pH 7.8, 25°C Leishmania major
0.192
-
 alpha-D-glucose 1-phosphate pH 7.8, 25°C Leishmania major
0.2
-
 diphosphate pH 7.8, 25°C Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54500
-
 1 * 54500, calculated and SDS-PAGE Leishmania major
59000
-
 gel filtration Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major Q4QDU3
-
-

Reaction

Reaction Comment Organism Reaction ID
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose ordered bi-bi mechanism. The molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate Leishmania major
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-glucose
-
 Leishmania major UTP + alpha-D-glucose 1-phosphate
-
 r
additional information molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate Leishmania major ?
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Leishmania major diphosphate + UDP-glucose
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 54500, calculated and SDS-PAGE Leishmania major