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Literature summary for 2.7.7.9 extracted from
- Identification of a dTDP-rhamnose biosynthetic pathway that oscillates with the molting cycle in Caenorhabditis elegans (2016), Biochem. J., 473, 1507-1521 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Caenorhabditis elegans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00947 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans |  |
| 0.01385 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.02422 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.03224 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.04988 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 0.07765 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | Caenorhabditis elegans | - |
diphosphate + dTDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | Caenorhabditis elegans | - |
diphosphate + UDP-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q18910 | - |
- |
| Caenorhabditis elegans | Q9XUS5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Caenorhabditis elegans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| body wall muscle | during larval development and in adult worm | Caenorhabditis elegans | - |
| hypodermis | during larval development | Caenorhabditis elegans | - |
| intestine | during larval development and in adult worm | Caenorhabditis elegans | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + ADP-alpha-D-glucose | - |
? | |
| CTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + CDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | - |
Caenorhabditis elegans | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| dTTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + dTDP-alpha-D-glucose | - |
? | |
| GTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + GDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | - |
Caenorhabditis elegans | diphosphate + UDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | RML-1 demonstrates a broad substrate range toward five nucleotides (dTTP, UTP, CTP, ATP and GTP) in the presence of alpha-D-glucose 1-phosphate, with higher activity toward dTTP and UTP | Caenorhabditis elegans | diphosphate + UDP-alpha-D-glucose | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | UGP-1 is specifically active toward UTP with alpha-D-glucose 1-phosphate, but displays very low or no activity toward other nucleotides, with either alpha-D-glucose 1-phosphate or alpha-D-mannose 1-phosphate | Caenorhabditis elegans | diphosphate + UDP-alpha-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| rml-1 | - |
Caenorhabditis elegans |
| UDP-Glc pyrophosphorylase | - |
Caenorhabditis elegans |
| ugp-1 | - |
Caenorhabditis elegans |
| UTP/dTTP-glucose-1-phosphate uridylyl/thymidylyl transferase | - |
Caenorhabditis elegans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 20.02 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 21.92 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 22.96 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 23.24 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 23.58 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 28.2 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | knockdown of rml-1, rml-4 and rml-5 (but not rml-2 and rml-3) causes lethality of the embryo | Caenorhabditis elegans |
| metabolism | rml-1 and gmp-1 are essential in embryogenesis and larval development | Caenorhabditis elegans |
| metabolism | the enzyme is involved in the dTDP-rhamnose biosynthetic pathway | Caenorhabditis elegans |
| metabolism | the enzyme is proposed to function in the biosynthesis of trehalose, one component of the maradolipids in the cuticle, which protectworms from harsh environments | Caenorhabditis elegans |
| metabolism | UTP/dTTP-glucose-1-phosphate uridylyl/thymidylyl transferase RML-1 converts alpha-D-glucose 1-phosphate in the presence of dTTP or UTP into dTDP-Glc or UDP-Glc, respectively. The dTDP-Glc is used for the biosynthesis of rhamnose in Caenorhabditis elegans, whereas the UDP-Glc may be used in the biosynthesis of glycogen, glycolipids and/or N-glycans | Caenorhabditis elegans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 1.45 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 470 | - |
alpha-D-glucose 1-phosphate | cosubstrate: dTTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 720 | - |
alpha-D-glucose 1-phosphate | cosubstrate: UTP, pH 7.5, 25°C | Caenorhabditis elegans | |
| 1160 | - |
dTTP | pH 7.5, 25°C | Caenorhabditis elegans | |
| 2310 | - |
UTP | pH 7.5, 25°C | Caenorhabditis elegans | |
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Release 2023.1 (January 2023)
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