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Literature summary for 2.7.7.9 extracted from

  • Kumpf, A.; Kowalczykiewicz, D.; Szymanska, K.; Mehnert, M.; Bento, I.; Lochowicz, A.; Pollender, A.; Jarzebski, A.; Tischler, D.
    Immobilization of the highly active UDP-glucose pyrophosphorylase from Thermocrispum agreste provides a highly efficient biocatalyst for the production of UDP-glucose (2020), Front. Bioeng. Biotechnol., 8, 740 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.9

Application

Application Comment Organism
synthesis immobilization enzyme provides a highly efficient biocatalyst for the production of UDP-glucose Thermocrispum agreste

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermocrispum agreste

Inhibitors

Inhibitors Comment Organism Structure
UTP
-
 Thermocrispum agreste

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
 UTP pH 7.0, 50°C Thermocrispum agreste
0.44
-
 alpha-D-glucose 1-phosphate pH 7.0, 50°C Thermocrispum agreste

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate Thermocrispum agreste
-
 diphosphate + UDP-glucose
-
 ?
UTP + alpha-D-glucose 1-phosphate Thermocrispum agreste DSM 44070
-
 diphosphate + UDP-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Thermocrispum agreste A0A2W4LV58
-
-
Thermocrispum agreste DSM 44070 A0A2W4LV58
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermocrispum agreste

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UTP + alpha-D-glucose 1-phosphate
-
 Thermocrispum agreste diphosphate + UDP-glucose
-
 ?
UTP + alpha-D-glucose 1-phosphate
-
 Thermocrispum agreste DSM 44070 diphosphate + UDP-glucose
-
 ?

Synonyms

Synonyms Comment Organism
TaGalU
-
 Thermocrispum agreste
UDP-glucose pyrophosphorylase
-
 Thermocrispum agreste

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Thermocrispum agreste

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for up to 30 min with a maximum loss of activity of 65%. The immobilized enzyme is stable at 50°C for at least 120 min without a significant loss of activity Thermocrispum agreste

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
598
-
 alpha-D-glucose 1-phosphate pH 7.0, 50°C Thermocrispum agreste
914
-
 UTP pH 7.0, 50°C Thermocrispum agreste

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.9
-
 UTP pH 7.0, 50°C Thermocrispum agreste

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1360
-
 alpha-D-glucose 1-phosphate pH 7.0, 50°C Thermocrispum agreste
6090
-
 UTP pH 7.0, 50°C Thermocrispum agreste