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Literature summary for 2.7.8.26 extracted from

  • Cameron, B.; Blanche, F.; Rouyez, M.C.; Bisch, D.; Famechon, A.; Couder, M.; Cauchois, L.; Thibaut, D.; Debussche, L.; Crouzet, J.
    Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5-phosphate) synthase (1991), J. Bacteriol., 173, 6066-6073.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of truncated CobV missing the first 20 codons in Escherichia coli Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0009
-
 adenosylcobinamide-GDP 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)
0.0027
-
 alpha-ribazole 5'-phosphate 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)
0.0078
-
 alpha-ribazole 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosylcobinamide-GDP + alpha-ribazole Pseudomonas denitrificans (nom. rej.)
-
 GMP + adenosylcobalamin
-
 ?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate Pseudomonas denitrificans (nom. rej.)
-
 GMP + adenosylcobalamin 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas denitrificans (nom. rej.) P29936
-
-

Purification (Commentary)

Purification (Comment) Organism
Superose 12 HR, Phenyl-Superose, partially purified Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosylcobinamide-GDP + alpha-ribazole
-
 Pseudomonas denitrificans (nom. rej.) GMP + adenosylcobalamin
-
 ?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
-
 Pseudomonas denitrificans (nom. rej.) GMP + adenosylcobalamin 5'-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CobV
-
 Pseudomonas denitrificans (nom. rej.)