BRENDA - Enzyme Database
show all sequences of 2.7.8.26

Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5-phosphate) synthase

Cameron, B.; Blanche, F.; Rouyez, M.C.; Bisch, D.; Famechon, A.; Couder, M.; Cauchois, L.; Thibaut, D.; Debussche, L.; Crouzet, J.; J. Bacteriol. 173, 6066-6073 (1991)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of truncated CobV missing the first 20 codons in Escherichia coli
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0009
-
adenosylcobinamide-GDP
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
0.0027
-
alpha-ribazole 5'-phosphate
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
0.0078
-
alpha-ribazole
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
Pseudomonas denitrificans (nomen rejiciendum)
-
GMP + adenosylcobalamin
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
Pseudomonas denitrificans (nomen rejiciendum)
-
GMP + adenosylcobalamin 5'-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas denitrificans (nomen rejiciendum)
P29936
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Superose 12 HR, Phenyl-Superose, partially purified
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
adenosylcobinamide-GDP + alpha-ribazole
-
489779
Pseudomonas denitrificans (nomen rejiciendum)
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
-
489779
Pseudomonas denitrificans (nomen rejiciendum)
GMP + adenosylcobalamin 5'-phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CobV
-
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of truncated CobV missing the first 20 codons in Escherichia coli
Pseudomonas denitrificans (nomen rejiciendum)
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0009
-
adenosylcobinamide-GDP
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
0.0027
-
alpha-ribazole 5'-phosphate
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
0.0078
-
alpha-ribazole
30°C, pH 9.0
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
Pseudomonas denitrificans (nomen rejiciendum)
-
GMP + adenosylcobalamin
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
Pseudomonas denitrificans (nomen rejiciendum)
-
GMP + adenosylcobalamin 5'-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Superose 12 HR, Phenyl-Superose, partially purified
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
-
489779
Pseudomonas denitrificans (nomen rejiciendum)
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
-
489779
Pseudomonas denitrificans (nomen rejiciendum)
GMP + adenosylcobalamin 5'-phosphate
-
-
-
?
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674322
Zayas
Reassessment of the late steps ...
Salmonella enterica
J. Bacteriol.
189
2210-2218
2007
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-
1
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-
-
-
-
-
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1
-
3
-
-
-
-
-
-
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3
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3
-
-
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-
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-
-
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-
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1
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1
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-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674341
Zayas
The CbiB protein of Salmonella ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
189
7697-7708
2007
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-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
8
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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2
-
-
-
1
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
662778
Maggio-Hall
The last step in coenzyme B(12 ...
Methanothermobacter thermautotrophicus, Salmonella enterica
Microbiology
150
1385-1395
2004
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-
1
-
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-
-
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2
-
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10
-
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1
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2
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-
-
-
-
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-
-
-
-
-
-
-
-
-
644450
Maggio-Hall
In vitro synthesis of the nucl ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
96
11798-11803
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
1
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
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2
-
-
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1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656416
Abd-Rahman
-
Gene cloning and sequence anal ...
Pyrococcus sp., Pyrococcus sp. KOD1
J. Ferment. Bioeng.
83
109-112
1997
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-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
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-
-
-
-
-
2
1
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-
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-
-
-
-
-
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1
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1
2
-
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-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489779
Cameron
Genetic analysis, nucleotide s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6066-6073
1991
-
-
1
-
-
-
-
3
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
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-
-
-
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-
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