BRENDA - Enzyme Database
show all sequences of 2.7.8.26

In vitro synthesis of the nucleotide loop of cobalamin by Salmonella typhimurium enzymes

Maggio-Hall, L.A.; Escalante-Semerena, J.C.; Proc. Natl. Acad. Sci. USA 96, 11798-11803 (1999)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression of His-tagged CobS in Escherichia coli; His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3)
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
Salmonella enterica subsp. enterica serovar Typhimurium
-
GMP + adenosylcobalamin
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
Salmonella enterica subsp. enterica serovar Typhimurium
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
adenosylcobalamin + GMP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
; strains JE212 and JE2197
-
Purification (Commentary)
Purification (Commentary)
Organism
; nickel affinity chromatography, recombinant CobS
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.008
-
recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
adenosylcobinamide-GDP + alpha-ribazole
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
adenosylcobalamin + GMP
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
?
adenosylcobinamide-GDP + alpha-ribazole
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
644450
Salmonella enterica subsp. enterica serovar Typhimurium
adenosylcobalamin + GMP
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
?
Synonyms
Synonyms
Commentary
Organism
cobalamin synthase
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobS
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of His-tagged CobS in Escherichia coli; His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3)
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
Salmonella enterica subsp. enterica serovar Typhimurium
-
GMP + adenosylcobalamin
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
Salmonella enterica subsp. enterica serovar Typhimurium
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
adenosylcobalamin + GMP
-
Salmonella enterica subsp. enterica serovar Typhimurium
?
Purification (Commentary) (protein specific)
Commentary
Organism
; nickel affinity chromatography, recombinant CobS
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.008
-
recombinant enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
adenosylcobinamide-GDP + alpha-ribazole
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
GMP + adenosylcobalamin
-
-
-
?
adenosylcobinamide-GDP + alpha-ribazole
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
adenosylcobalamin + GMP
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
?
adenosylcobinamide-GDP + alpha-ribazole
de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin
644450
Salmonella enterica subsp. enterica serovar Typhimurium
adenosylcobalamin + GMP
-
644450
Salmonella enterica subsp. enterica serovar Typhimurium
?
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674322
Zayas
Reassessment of the late steps ...
Salmonella enterica
J. Bacteriol.
189
2210-2218
2007
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674341
Zayas
The CbiB protein of Salmonella ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
189
7697-7708
2007
-
-
-
-
-
-
-
-
-
-
-
2
-
4
-
-
1
-
-
-
-
-
8
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
8
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
662778
Maggio-Hall
The last step in coenzyme B(12 ...
Methanothermobacter thermautotrophicus, Salmonella enterica
Microbiology
150
1385-1395
2004
-
-
1
-
-
-
-
-
2
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644450
Maggio-Hall
In vitro synthesis of the nucl ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
96
11798-11803
1999
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
-
-
1
-
3
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656416
Abd-Rahman
-
Gene cloning and sequence anal ...
Pyrococcus sp., Pyrococcus sp. KOD1
J. Ferment. Bioeng.
83
109-112
1997
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
489779
Cameron
Genetic analysis, nucleotide s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6066-6073
1991
-
-
1
-
-
-
-
3
-
-
-
2
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-