Cloned (Comment) | Organism |
---|---|
expression of His-tagged CobS in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
His-tagged CobS expressed in Escherichia coli BL21(lambdaDE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide-GDP + alpha-ribazole | Salmonella enterica subsp. enterica serovar Typhimurium | - |
GMP + adenosylcobalamin | - |
? | |
adenosylcobinamide-GDP + alpha-ribazole | Salmonella enterica subsp. enterica serovar Typhimurium | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin | adenosylcobalamin + GMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
strains JE212 and JE2197 | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
nickel affinity chromatography, recombinant CobS | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | - |
recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide-GDP + alpha-ribazole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | GMP + adenosylcobalamin | - |
? | |
adenosylcobinamide-GDP + alpha-ribazole | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobalamin + GMP | - |
? | |
adenosylcobinamide-GDP + alpha-ribazole | de novo synthesis of adenosylcobalamin under anaerobic conditions, nucleotide loop assembly pathway, final step in adenosylcobalamin biosynthesis, condensation of AdoCbi-GDP with alpha-ribazole to yield adenosylcobalamin | Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobalamin + GMP | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cobalamin synthase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobS | - |
Salmonella enterica subsp. enterica serovar Typhimurium |