BRENDA - Enzyme Database
show all sequences of 2.7.8.26

The CbiB protein of Salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway

Zayas, C.L.; Claas, K.; Escalante-Semerena, J.C.; J. Bacteriol. 189, 7697-7708 (2007)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
Salmonella enterica
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
GMP + ?
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
Salmonella enterica LT2
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
GMP + ?
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Salmonella enterica
-
serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
-
Salmonella enterica LT2
-
serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
-
Purification (Commentary)
Purification (Commentary)
Organism
solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
Salmonella enterica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
alpha-ribazole + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica
GMP + adenosylcobalamin
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica LT2
GMP + adenosylcobalamin
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica
GMP + adenosylcobalamin 5'-phosphate
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica LT2
GMP + adenosylcobalamin 5'-phosphate
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
674341
Salmonella enterica
GMP + ?
-
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
674341
Salmonella enterica LT2
GMP + ?
-
-
-
?
additional information
about 100fold more efficient with alpha-ribazole-5-phosphate as substrate than using alpha-ribazole, in vivo
674341
Salmonella enterica
?
-
-
-
-
additional information
about 100fold more efficient with alpha-ribazole-5-phosphate as substrate than using alpha-ribazole, in vivo
674341
Salmonella enterica LT2
?
-
-
-
-
Synonyms
Synonyms
Commentary
Organism
adenosylcobalamin synthase
-
Salmonella enterica
AdoCbl-5-P synthase
-
Salmonella enterica
CobS
-
Salmonella enterica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.67
-
alpha-ribazole
-
Salmonella enterica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
Salmonella enterica
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
GMP + ?
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
Salmonella enterica LT2
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
GMP + ?
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+))
Salmonella enterica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
alpha-ribazole + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica
GMP + adenosylcobalamin
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica LT2
GMP + adenosylcobalamin
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica
GMP + adenosylcobalamin 5'-phosphate
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP
1 h, 37C, pH 9, in dim light, equimolar substrate concentration
674341
Salmonella enterica LT2
GMP + adenosylcobalamin 5'-phosphate
reaction stop by KCN and incubation 10 min at 80C, analysis by reverse-phase HPLC
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
674341
Salmonella enterica
GMP + ?
-
-
-
?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP
second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5-phosphate by alpha-ribazole-phosphate phosphatase (CobC)
674341
Salmonella enterica LT2
GMP + ?
-
-
-
?
additional information
about 100fold more efficient with alpha-ribazole-5-phosphate as substrate than using alpha-ribazole, in vivo
674341
Salmonella enterica
?
-
-
-
-
additional information
about 100fold more efficient with alpha-ribazole-5-phosphate as substrate than using alpha-ribazole, in vivo
674341
Salmonella enterica LT2
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.67
-
alpha-ribazole
-
Salmonella enterica
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
674322
Zayas
Reassessment of the late steps ...
Salmonella enterica
J. Bacteriol.
189
2210-2218
2007
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-
1
-
-
-
-
-
-
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1
-
3
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-
-
-
-
-
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3
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3
-
-
-
-
-
-
-
-
-
-
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-
1
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-
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1
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-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674341
Zayas
The CbiB protein of Salmonella ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
189
7697-7708
2007
-
-
-
-
-
-
-
-
-
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2
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4
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1
-
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8
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3
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-
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1
-
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2
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1
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8
-
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-
1
-
-
-
-
-
-
-
-
-
-
662778
Maggio-Hall
The last step in coenzyme B(12 ...
Methanothermobacter thermautotrophicus, Salmonella enterica
Microbiology
150
1385-1395
2004
-
-
1
-
-
-
-
-
2
-
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-
-
10
-
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-
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-
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1
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2
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644450
Maggio-Hall
In vitro synthesis of the nucl ...
Salmonella enterica subsp. enterica serovar Typhimurium
Proc. Natl. Acad. Sci. USA
96
11798-11803
1999
-
-
1
-
-
-
-
-
-
-
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2
-
1
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1
-
-
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1
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3
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2
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1
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2
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1
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1
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3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656416
Abd-Rahman
-
Gene cloning and sequence anal ...
Pyrococcus sp., Pyrococcus sp. KOD1
J. Ferment. Bioeng.
83
109-112
1997
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1
-
-
-
-
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1
2
-
6
-
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-
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2
1
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1
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1
2
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2
1
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-
-
-
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-
-
-
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-
-
-
-
489779
Cameron
Genetic analysis, nucleotide s ...
Pseudomonas denitrificans (nomen rejiciendum)
J. Bacteriol.
173
6066-6073
1991
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1
-
-
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3
-
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2
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1
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1
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2
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1
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1
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3
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2
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1
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2
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