Cloned (Comment) | Organism |
---|---|
sfp and acpS cloned and overproduced in Escherichia coli M15 | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
co-crystallized with CoA and ACP | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate | Bacillus subtilis | |
0.0014 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate | Bacillus subtilis | |
0.00445 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme Sfp | Bacillus subtilis | |
0.0216 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP | Bacillus subtilis | |
0.026 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP | Bacillus subtilis | |
0.038 | - |
apo-ACP | pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate | Bacillus subtilis | |
0.068 | - |
apo-ACP | pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Bacillus subtilis | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant isoenzymes AcpS and Sfp, expressed in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[peptidyl carrier protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein] | - |
r | |
additional information | Sfp type exhibits an extraordinarily broad substrate specificity | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | isoenzyme AcpS | Bacillus subtilis |
trimer | isoenzyme Sfp | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0283 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate | Bacillus subtilis | |
0.208 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate | Bacillus subtilis | |
0.239 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP | Bacillus subtilis | |
0.367 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate | Bacillus subtilis | |
1.6 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme Sfp | Bacillus subtilis | |
1.6 | - |
apo-peptidyl carrier protein | pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP | Bacillus subtilis | |
2.08 | - |
apo-acyl-carrier protein | pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate | Bacillus subtilis |