Cloned (Comment) | Organism |
---|---|
gene npgA, DNA and amino acid sequence determination and analysis, recombinant overexpression of His6-tagged enzyme in Aspergillus nidulans npgA knockout strain SNT611 using the niiA promoter, and as His6-tagged enzyme in Escherichia coli, functional complementation of Saccharomyces cerevisiae strain BY4741 knockout mutant DPNG | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a npgA knockout mutant in strain SNT611 | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aspergillus nidulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37700 | - |
- |
Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Aspergillus nidulans | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Aspergillus nidulans SNT611 | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q9UVK7 | - |
- |
Aspergillus nidulans SNT611 | Q9UVK7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Aspergillus nidulans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cleistothecium | - |
Aspergillus nidulans | - |
conidium | - |
Aspergillus nidulans | - |
mycelium | - |
Aspergillus nidulans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Aspergillus nidulans | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTase cleaves coenzyme A, transfers the P-pant moiety to a conserved residue of inactive substrates, and produces 3'5'-ADP | Aspergillus nidulans | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | - |
Aspergillus nidulans SNT611 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTase cleaves coenzyme A, transfers the P-pant moiety to a conserved residue of inactive substrates, and produces 3'5'-ADP | Aspergillus nidulans SNT611 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 37700, recombinant His6-tagged enzyme, SDS-PAGE | Aspergillus nidulans |
More | enzyme NpgA contains a PPTase superfamily domain, which has a conserved sequence of (V/I)G(V/I)DX(78)(F/W)(S/C/T)XKE(A/S)hhK sequence between aa 187-277, similar to PPTases of other organisms | Aspergillus nidulans |
Synonyms | Comment | Organism |
---|---|---|
4'-phosphopantetheinyl transferase | - |
Aspergillus nidulans |
NpgA | - |
Aspergillus nidulans |
P-pant transferase | - |
Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Aspergillus nidulans |
General Information | Comment | Organism |
---|---|---|
malfunction | pleiomorphic phenomena of npgA1 mutant, phenotype, overview | Aspergillus nidulans |
additional information | loss-of-function of LYS5 mutant DNPG, which encodes a PPTase in Saccharomyces cerevisiae strain BY4741, is functionally complemented by NpgA, and Escherichia coli-derived NpgA reveals phosphopantetheinylation activity with the elaboration of 3'5'-ADP | Aspergillus nidulans |
physiological function | the enzyme is essential for growth, and for formation of conidia and cleistothecium in development | Aspergillus nidulans |