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Literature summary for 2.7.9.1 extracted from

  • Rivero, L.A.; Concepcion, J.L.; Quintero-Troconis, E.; Quinones, W.; Michels, P.A.; Acosta, H.
    Trypanosoma evansi contains two auxiliary enzymes of glycolytic metabolism: phosphoenolpyruvate carboxykinase and pyruvate phosphate dikinase (2016), Exp. Parasitol., 165, 7-15.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma evansi 20015
-
additional information the enzyme is released from whole trypomastigotes upon addition of digitonin at increasing concentrations Trypanosoma evansi
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Trypanosoma evansi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate + phosphate Trypanosoma evansi
-
AMP + phosphoenolpyruvate + diphosphate
-
r
ATP + pyruvate + phosphate Trypanosoma evansi TEVA 1
-
AMP + phosphoenolpyruvate + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Trypanosoma evansi
-
isolated from a horse
-
Trypanosoma evansi TEVA 1
-
isolated from a horse
-

Purification (Commentary)

Purification (Comment) Organism
purification of glycosomes, the enzyme is released from whole trypomastigotes upon addition of digitonin at increasing concentrations Trypanosoma evansi

Source Tissue

Source Tissue Comment Organism Textmining
trypomastigote Trypanosoma evansi does not undergo stage-dependent differentiations, it occurs only as bloodstream form Trypanosoma evansi
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.016
-
purified glycosomes, pH 7.2, 28°C Trypanosoma evansi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Trypanosoma evansi AMP + phosphoenolpyruvate + diphosphate
-
r
ATP + pyruvate + phosphate
-
Trypanosoma evansi TEVA 1 AMP + phosphoenolpyruvate + diphosphate
-
r

Synonyms

Synonyms Comment Organism
PPDK
-
Trypanosoma evansi
pyruvate phosphate dikinase
-
Trypanosoma evansi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
assay at Trypanosoma evansi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Trypanosoma evansi

Cofactor

Cofactor Comment Organism Structure
AMP
-
Trypanosoma evansi
ATP
-
Trypanosoma evansi

General Information

General Information Comment Organism
evolution Trypanosoma evansi does not undergo stage-dependent differentiations, it occurs only as bloodstream forms, the metabolic pattern of this parasite is not identical to that of the bloodstream form of Trypanosoma brucei, modelling, overview Trypanosoma evansi
metabolism the enzyme PPDK is an auxiliary enzyme of glycolytic metabolism present in glycosomes, modelling, overview Trypanosoma evansi
physiological function the enzyme plays an important role in the maintenance of the ATP/ADP balance in glycosomes in this life-cycle stage of the parasite, as well as contributes in part to its glycolytic flux Trypanosoma evansi