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Literature summary for 2.7.9.2 extracted from
- Phosphoenolpyruvate synthetase of Escherichia coli. Purification, some properties, and the role of divalent metal ions (1970), J. Biol. Chem., 245, 5309-5318.
General Stability
| General Stability | Organism |
|---|---|
| glycerol stabilizes the purified enzyme | Escherichia coli |
| KCl destabilizes | Escherichia coli |
| sucrose, 1.0 M, stabilizes against inactivation by heat, acidic pH, and during storage | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | excess of ATP inhibits at high concentrations of MgCl2 or MnCl2 | Escherichia coli |  |
| Ca2+ | inhibits Mn2+-activated enzyme | Escherichia coli | |
| iodoacetate | - |
Escherichia coli | |
| Mg2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction, inhibition at high concentrations of Mg2+ or Mn2+ | Escherichia coli | |
| Mn2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction, inhibition at high concentrations of Mg2+ or Mn2+ | Escherichia coli | |
| PCMB | - |
Escherichia coli | |
| sulfhydryl reagents | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| 0.028 | - |
ATP | pH 8.0, 25°C | Escherichia coli | |
| 0.083 | - |
pyruvate | pH 8.0, 25°C | Escherichia coli | |
| 10.5 | - |
phosphate | pH 6.8, 23°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction | Escherichia coli | |
| Mg2+ | inhibition at high concentrations of Mg2+ or Mn2+ | Escherichia coli | |
| Mn2+ | 3 to 4 mol of Mn2+ bound per mol of enzyme | Escherichia coli | |
| Mn2+ | divalent metal ion Mg2+ or Mn2+ required for forward reaction | Escherichia coli | |
| Mn2+ | inhibition at high concentrations of Mg2+ or Mn2+ | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.9 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 10 mM Tris-HCl buffer, pH 6.8, containing 1 M sucrose, 0.2 mM EDTA, 0.2 mM dithioerythritol, no loss of activity after 1 year | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + H2O | - |
Escherichia coli | AMP + phosphoenolpyruvate + phosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
20 min, 17% remaining activity, in presence of 1 M sucrose: 96% remaining activity | Escherichia coli |
| 70 | - |
10 min, no remaining activity | Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.8 | purified enzyme, most stable at, rapid loss of activity above pH 6.8 and below pH 5.5 | Escherichia coli |
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